MEDICATION FOR DIAGNOSTICS OF CORNEAL EPITHELIAL DAMAGE

Abstract

To study the integrity of corneal epithelium in case of injuries and damages of the cornea solution of fluorescein and less frequently rose Bengal are traditionally used. However, these drugs are not always available for the specialists. Therefore, to identify defects of the corneal epithelium collargol or Riboflavin as dyes were started to be used. The aim of this study was to investigate the possibility of applying a solution of Riboflavin with hydroxypropyl methylcellulose to diagnose damage to the corneal epithelium. The experiment studied a designed eye care product containing a 1% solution of Riboflavin mononucleotide with 1% hydroxypropyl methylcellulose (HPMC), compared with a 1% solution of fluorescein (control) and 1% solution of Riboflavin mononucleotide and 20% dextran.The study revealed a high coloring ability of the polymer-containing solutions of Riboflavin with dextran and HPMC in comparison with an aqueous fluorescein, which is associated with the formation of the stable film on the surface of the cornea. Comparative study of medications with Riboflavin / dextran and Riboflavin / HPMC showed greater effectiveness of the latter due to stable precorneal coloring film because of the viscosity of the solution and the optimal concentration of Riboflavin mononucleotide in the layers of the anterior stroma. However, there was no dehydrogenating effect of the cornea due to the dehydrating action of a solution of Riboflavin with dextran. Thus, the use of eye care products that contain 1% of Riboflavin mononucleotide and 1% HPMC provides effective and long-lasting biomicroscopy for damaged areas of the corneal epithelium.