STUDY OF MORPHOLOGY AND MICROSCOPIC CHARACTERISTICS AND QUALITATIVE ANALYSIS OF SALVIA STEPPOSA SHOST.
Abstract
The aim of this study is to conduct macroscopic, microscopic and qualitative analysis of Salvia stepposa.
Material and methods. The research studied the grass and leaves of Salvia stepposa, harvested on the territory of the Republic of Bashkortostan during the flowering period of the plant in July 2022. Macroscopic and microscopic analysis was carried out using methods described in regulatory documentation [6]. Pharmacological chemical reactions for flavonoids and phenolic compounds were carried out with the obtained alcoholic extracts. The same groups of substances were studied by thin layer chromatography. Thin layer chromatography was carried out on Sorbfil PTSH-AF-A 100×150 mm plates in the system of ethyl acetate - formic acid - water (15:3:4) using standard samples of flavonoids and hydroxycinnamic acids.
Results. Diagnostically significant morphological characteristics of the plant under study have been established, which can be used to identify raw materials. Experiments have shown that the raw material contains flavonoid compounds such as rutin, luteolin7-glycoside, hyperoside and also phenolic compounds — caffeic and chlorogenic acids. Chromatographic study suggested that luteolin-7-glycoside is the main flavonoid of this plant. The conducted research can be used to develop regulatory documentation for S. stepposa raw materials.
About the Authors
K. I. Kashfullina
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
S. R. Khasanova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
N. V. Kudashkina
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
J. S. Toksanbayeva
АО «Южно-Казахстанская медицинская академия»
Kazakhstan
Kazakhstan
T. V. Bulgakov
ФГБОУ ВО «Московский государственный университет имени М.В. Ломоносова»
Russian Federation
Russian Federation
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Review
For citations:
Kashfullina K.I., Khasanova S.R., Kudashkina N.V., Toksanbayeva J.S., Bulgakov T.V. STUDY OF MORPHOLOGY AND MICROSCOPIC CHARACTERISTICS AND QUALITATIVE ANALYSIS OF SALVIA STEPPOSA SHOST. Bashkortostan Medical Journal. 2024;19(3):47-52. (In Russ.)
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