INVESTIGATION OF ACUTE ORAL TOXICITY OF NEW DERIVATIVES OF CONDENSED 3-AMINOTHIENO[2,3-B]PYRIDINES AND 1,4-DIHYDROPYRIDINES WITH POTENTIAL ANALGESIC ACTIVITY

New compounds from cyanothioacetamide derivatives are of particular interest to pharmaceutical and medical scientists. We have previously established that certain derivatives of fused 3-aminothieno[2,3-b]pyridines and 1,4-dihydropyridines have pronounced antiexudative activity with analgesic properties.

Purpose of research. The study of acute oral toxicity of new condensed derivatives of thienopyridine and 1,4-dihydropyridine synthesized by us, the most promising in terms of creating analgesics.

Material and methods. The study was conducted in accordance with the Interstate Standard GOST 32644-2014 (OECD, Test № 423:2001, IDT) on 60 white laboratory female rats weighing 180-220 g. Compounds in the form of an aqueous suspension were administered once intragastrically at doses of 5, 50, 300, and 2000 mg/kg.

Results. For the entire period of observation, not a single case of death of rats of the experimental groups was registered in any of the indicated dosages. Zero lethality of rats treated with new derivatives of 3-aminothieno[2,3-b]pyridine and 1,4-dihydropyridine with laboratory codes AZ023, AZ331, AZ420 and AZ383 at a dose of 2000 mg/kg allowed them to be assigned to the 5th toxicity class. LD50 for new derivatives of thienopyridine and 1,4-dihydropyridine is >5000 mg/kg.

Conclusion. Four studied derivatives of new heterocyclic compounds containing 3-aminothieno[2,3-b]pyridine and 1,4-dihydropyridine fragments with AZ023, AZ331, AZ420 and AZ383 laboratory codes belong to the 5th toxicity class (low toxicity compounds).

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