Association of RS25487 and RS415407 polymorphism of the XRCC1 DNA repair system protein gene with the risk of developing autopathic infertility
Abstract
The aim: to study the role of DNA repair system gene polymorphisms XRCC1 rs25487 and rs415407 as risk factors for male infertility.
Material and methods. The study was conducted at the ART clinic «Family» in Ufa. 197 men of reproductive age were examined. The main group consisted of 112 infertile patients, the comparison group – 85 fertile men. Sperm analysis was performed in accordance with the WHO protocol (2010). DNA was isolated from sperm using the QIAamp DNA Micro Kit. Determination of the genotypes of polymorphic loci gene XRCC1 was performed using the TaqMan allele discrimination method. Allelic discrimination analysis was performed on the CFX96 Real-Time PCR Detection System. The results of allelic discrimination were analyzed using the CFX96 Real-Time PCR Detection System software.
Results. It was found that the rs25487*GG genotype and rs25487*G allele are markers of an increased risk of autopathic male infertility, while the rs25487*AA genotype and rs25487*A allele are associated with a reduced risk of decreased fertility.
Conclusion. Polymorphisms of the genes of the DNA repair system are associated with the reproductive status of men. The obtained data are evidence that polymorphic variants of the XRCC1 protein gene contribute to an increased risk of pathospermia.
About the Authors
E. F. Galimova
Bashkir State Medical University Ministry of Health of Russia
Russian Federation
Russian Federation
Ufa
Yu. Yu. Gromenko
OOO "Medical center "Family"
Russian Federation
Russian Federation
Ufa
A. A. Baigildina
St. Joseph University (SJUIT), College of health and allied sciences
United Republic of Tanzania
United Republic of Tanzania
Dar es Salaam
K. Sh. Galimov
Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Moscow
P. F. Litvitsky
Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Moscow
K. A. Bikmetov
Bashkir State Medical University Ministry of Health of Russia
Russian Federation
Russian Federation
Ufa
Sh. N. Galimov
Bashkir State Medical University Ministry of Health of Russia
Russian Federation
Russian Federation
Ufa
V. N. Pavlov
Bashkir State Medical University Ministry of Health of Russia
Russian Federation
Russian Federation
Ufa
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Review
For citations:
Galimova E.F., Gromenko Yu.Yu., Baigildina A.A., Galimov K.Sh., Litvitsky P.F., Bikmetov K.A., Galimov Sh.N., Pavlov V.N. Association of RS25487 and RS415407 polymorphism of the XRCC1 DNA repair system protein gene with the risk of developing autopathic infertility. Bashkortostan Medical Journal. 2024;19(5):15-19. (In Russ.)
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