Morphological changes in lymphoid structures of the left and right liver ducts in mechanical cholestasis under experiment
Abstract
Cholestasis leads to the development of biochemical, physiological and morphological changes. Therefore, accurate information about the morphology of the structural components of the biliary tracts is necessary for cholestasis.
Aim. To evaluate the structural and morphometric characteristics of the lymphoid formations of the left and right hepatic ducts of animals after mechanical blockade of the common hepatic duct.
Material and methods. The study was conducted on 30 rabbits of no specific breed (15 in the main group, 15 in the control group). By taking the common hepatic duct into a ligature, a model of cholestasis was created. For macromicroscopic examination, pieces of the left and right hepatic ducts of rabbits from the experimental and control groups were taken. The prepared sections were stained with hematoxylin-eosin, methylene blue, and the Van Gieson method. Lymphoid nodules on whole preparations were studied using the Hellman T method.
Results. According to our data, after mechanical blockage of the common hepatic duct, in the walls of the left and right hepatic ducts swelling and stagnation in the veins are observed, the size and quantitative indicators of lymphoid structures decrease, the percentage of cells in a state of degeneration increases, large lymphocytes and cells with a pattern of mitosis are not observed.
References
1. Direktiva 2010/63/EU Evropejskogo Parlamenta i Evropejskogo Sojuza: Po ohrane zhivotnyh, ispol'zuemyh v nauchnyh celjah (Directive 2010/63/EU of the European Parliament and the European Union: On the protection of animals used for scientific purposes). St. Petersburg: Spets.Lit, 2012:48. (In Russ)
2. Korzhevsky, D.E. Morfologicheskaja diagnostika (Morphological diagnostics). St. Petersburg: Spets.Lit, 2016. 128.. (In Russ)
3. Makhmadov F.I., Ashurov A.S., Tagoikulov E.H. Liver morphology in regulated mechanical jaundice (experimental study). Healthcare in Tajikistan 2023; 2: 69-76 (In Russ)
4. Polunina T.E. Cholestasis: algorithms for diagnosis and treatment. Academy of medicine and sports. 2021;4::28-36 (In Russ)
5. Marinkin I.O., Sokolova T.M., Kiseleva T.V. [et al.]. Cholestasis of pregnancy. Experimental and clinical gastroenterology. 2016; 9:81-85. (In Russ)
6. Tsyrkunov V.M., Prokopchik N.I., Andreev V.P. Clinical morphology of liver: cholestasis. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta, 2018;4:468-480 (In Russ) doi: 10.25298/2221-8785-2018-16-4-468-480
7. Shadlinski V.B., Gasymova T.M., Nikityuk D.B. Morphological changes of lymphoid apparatus of the larynx after experimental exposure to various balneal factors. Morfologiya. 2014;2: 21-25. (In Russ)
8. Ananth R. Neonatal Cholestasis: A Primer of Selected Etiologies. Pediatr Ann. 2018;47(11):433-439. (In Engl)
9. Andrianov M.G., Azzam R.K. Cholestasis in Infancy. Pediatr Ann. 2016;45(12):e414-e419. (In Engl) doi: 10.3928/19382359-20161118-01.
10. García-Bermúdez V., Morales-Ortega A., Jaquero M.I. [et al.]. Primary liver lymphoma and systemic sclerosis. Oxford: Rheumatology. 2022, Aug 3; 61(8):e247-e248. doi: 10.1093/rheumatology/keac026
11. Hellman T. Studien uber das lymphoid Gewebe. Beit. Path. Anat. All. Pathol. 1921;68:333-363 (In German)
12. Huseynova G.A. Morphologıcal changes of the lymphoıd apparatus of urınary bladder of rats after experımental ınfluence of varıous balneologıcal factors. American Scientific Journal. 2020;38:20-25 (In Engl)
13. Gafarov I.A. Biostatistics. Bakı, 2021:238. (In Azer)
14. Ren W., Shi D. Experimental study on repair of bile duct defects with expanded polytetrafluoroethylene. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2011; 15(3): 305-307 (In Engl)
15. Shadlinskaya S.V. Features of the density of the location and changes in the cellular composition of the lymphoid formations of the vaginal vestibule of rats after a course of naphthalan baths. Journal of life sciences and biomedicine. 2019;1(1):83-86 (In Engl)
Review
For citations:
Alievа N.G. Morphological changes in lymphoid structures of the left and right liver ducts in mechanical cholestasis under experiment. Bashkortostan Medical Journal. 2024;19(5):29-34. (In Russ.)
Views:
42
Email this article 
