Histological and morphometric characteristics of the hippocampus of nonlinear white rats in different age periods
Abstract
Analysis of morphological processes accompanying aging in the hippocampus is one of the urgent problems of the present time.
Purpose. To assess morphofunctional state of pyramidal neurons in the hippocampus of nonlinear white rats.
Material and methods. Using histological and morphometric methods, we have studied autopsy material (70 hippocampi) obtained from animals of both sexes of 3 age categories.
Results. The results of the study showed the presence of signs of dystrophic changes in the pyramidal neurons of the main fields of the hippocampus in adult animals. However, in animals of the older age group, the number of degenerative changes in neurons increased. This statement was also supported by a significant decrease in the area of pyramidal cell bodies in the CA1, CA3 and CA4 fields of the hippocampus in old rats when compared with group II (adult animals), which was confirmed by morphometry (р < 0,05). In addition, cell sizes in old animals in all hippocampal fields corresponded to the sizes of neurons in 14-day-old animals.
About the Authors
N. A. Zimushkina
ФГБОУ ВО «Пермский государственный медицинский университет им. академика Е.А. Вагнера» Минздрава России
Russian Federation
Russian Federation
Yu. P. Torsunova
ФГБОУ ВО «Пермский государственный медицинский университет им. академика Е.А. Вагнера» Минздрава России
Russian Federation
Russian Federation
N. A. Loginova
ФГБОУ ВО «Пермский государственный медицинский университет им. академика Е.А. Вагнера» Минздрава России
Russian Federation
Russian Federation
P. A. Garyaev
ФГБОУ ВО «Пермский государственный медицинский университет им. академика Е.А. Вагнера» Минздрава России
Russian Federation
Russian Federation
E. V. Ponomarenko
ФГБОУ ВО «Пермский государственный медицинский университет им. академика Е.А. Вагнера» Минздрава России
Russian Federation
Russian Federation
References
1. Kulesh, A. M., Cherkasova V. G. Uroven' sRAGE i ego svyaz' s kognitivnym statusom pri bolezni Al'tsgeimera i smeshannykh sosudisto-degenerativnykh kognitivnykh narusheniyakh (Stage sRAGE and its relation to cognitive status in Alzheimer's disease and mixed vascular degenerative cognitive disorders). Uspekhi gerontologii. 2016; 29 (1): 159-163. (In Russ).
2. BogolepovaI. N., IllarioshkinS. N., SveshnikovA. V.[et.al]. Gendernye osobennosti stroeniya gippokampa mozga muzhchin i zhenshchin (Gender features of the structure of the hippocampus of the brain of men and women). Hurnal anatomii i gistopatologii. 2016; 5 (1): 15-19. (In Russ).https://doi.org/10.18499/2225-7357-2016-5-1-15-19
3. Mal'cevA. V., Dovidchenko N. V., Uteshev V. K. [et.al]. Puskovye factory amiloidoza nejronov i bolezni Al'cgejmera (Triggering factors of neuronal amyloidosis and Alzheimer's disease). Biomedicinskaya himiya. 2013; 59 (2): 144-170. (In Russ).
4. Hatamov A. I. Osobennosti citoarhitektoniki kory entorinal'noj oblasti mozga detej s rozhdeniya do 7 let (Features of the cytoarchitectonics of the cortex of the entorhinal region of the brain of children from birth to 7 years old). Morfologiya. Sankt-Peterburg. 2008; 133 (3): 144 .(In Russ).
5. Smirnov A. V., TyurenkovI.N., ShmidtM.V. [et.al]. Harakteristika morfologicheskih izmenenij gippokampa staryh krys v rezul'tate stressovogo vozdejstviya (Characteristics of morphological changes in the hippocampus of old rats as a result of stress exposure). Vestnik VolGMU. 2013; 2(46): 14-17. (In Russ).
6. Sengupta, P. The laboratory rat: Relating its age with human’s. Int J Prev Med. 2013; 4 (6): 624-30. (InEngl).
7. Paxinos, G. The Rat Brain in Stereotaxic Coordinates / G. Paxinos, S. Watson. Sydney. Academic Press. 1998: 237. (in Engl).
8. Knierim JJ. The hippocampus. Curr Biol. 2015; 25(23): 1116-21. (in Engl). doi: 10.1016/j.cub.2015.10.049.
9. Hudoerkov R. M. Metody komp'yuternoj morfometrii v nejromorfologii: uchebnoe posobie (bazovyj kurs) (Methods of computer morphometry in neuromorphology: a textbook (basic course)). FGBU «Nauchnyj centr nevrologii» RAMN. Moskva. 2014:53. (In Russ).
10. Zaidel D.W. Quantitative Morphology of Human Hippocampus Early Neuron Development. The Anatomical Record. 1999; 254: 87–91.(in Engl).
11. Malinovskaya N. A., Prokopenko S.V., Komleva YU.K. [et.al]. Molecules-markers of glial activation in neuroinflammation: new opportunities for pharmacotherapy of neurodegeneration. Siberian Medical Review. 2014; 5: 5-13. (In Russ).
12. Geerligs L., Renken R.J., Saliasi E.[et.al].A Brain-Wide Study of Age-Related Changes in Functional Connectivity. Cereb Cortex. 2015; 25(7):1987-99. (in Engl). doi: 10.1093/cercor/bhu012.
13. Zhabotinskii YU.M. Normal'naya i patologicheskaya morfologiya neirona (Normal and pathological morphology of the neuron). Akad. med. nauk SSSR. Leningrad: Meditsina. Leningr. otd-nie. 1965: 323. (In Russ).
14. Maksimova K.Yu., Stefanova N.A., Logvinov S.V. Morphological changes in the hippocampus of rats in accelerated aging. Bulletin of Siberian Medicine. 2014;13(1):56-61. (In Russ.) https://doi.org/10.20538/1682-0363-2014-1-56-61
15. Zimna A, Kurpisz M. Hypoxia-Inducible Factor-1 in Physiological and Pathophysiological Angiogenesis: Applications and Therapies. BiomedResInt. 2015;2015:549412. (in Engl). doi: 10.1155/2015/549412.
Review
For citations:
Zimushkina N.A., Torsunova Yu.P., Loginova N.A., Garyaev P.A., Ponomarenko E.V. Histological and morphometric characteristics of the hippocampus of nonlinear white rats in different age periods. Bashkortostan Medical Journal. 2025;20(1):62-66. (In Russ.)
Views:
21
Email this article 
