A method for epithelial cells isolation and cultivation from rabbit kidney
Abstract
The cubic epithelium of the kidney is an indispensable and widespread in vitro model for studying the physiology of mammalian cells. These cells play a key role in blood filtration, nutrient reabsorption and waste disposal, so their behavior directly affects kidney function. The use of renal epithelial cells in research makes it possible to study pathological processes at the cellular level, for example, the effect of toxins, drugs or genetic mutations on kidney function.
The aim of our work was to obtain a primary line of epithelial cells from a rabbit kidney.
Materials and methods. Primal cells were isolated through tissue dissociation in collagenase type 1, followed by cultivation in the media enriched with growth factors: human insulin, transferrin, dexamethasone and epidermal growth factor. The obtained cell subculture was verified by the immunocytochemical method. Anti-alpha 1 Sodium Potassium ATPase and N-cadherin were selected as tissue–specific markers of the cubic epithelium of the kidney, and alpha-SMA is a marker of smooth muscle cells and fibroblasts, was selected as a negative control. The enzyme Na+/K+-ATPase plays an important role in blood filtration and elimination of toxins in mammalian kidneys. N-cadherin is a protein that is involved in the formation of dense contacts of epithelial tissues.
Results: the isolated cells actively proliferated in a specialized culture medium and demonstrated morphological characteristics that are typical for epithelial phenotype. The results of immunofluorescence microscopy confirmed the epithelial origin of the cells. As a result, a primary stable line of the rabbit kidney epithelium was obtained, suitable for further experimental work in vitro.
About the Authors
T. I. Bikkuzin
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
R. R. Yazgarova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
A. V. Mikhailova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
Sh. R. Rakhmatullin
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
D. I. Khalilov
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Bikkuzin T.I., Yazgarova R.R., Mikhailova A.V., Rakhmatullin Sh.R., Khalilov D.I. A method for epithelial cells isolation and cultivation from rabbit kidney. Bashkortostan Medical Journal. 2025;20(1):66-71. (In Russ.)
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