EVALUATION OF IMMUNO-TOXICOLOGICAL PROPERTIES OF AROMATIC COMPOUNDS
Abstract
In this paper the effect of 2-methyl-4-chlorophenoxyacetic acid, p-tert-butylphenol, naphthalene, γ-naphthalenesulfonic on viability of HEK293 cells (human embryonic kidney cells) and human T-lymphocytes has been studied. Evaluation of action of these compounds on the immune function of human cells can be useful in terms of expansion of representations about potential immunotropic properties of above mentioned xenobiotics. The HEK293 cell line and mononuclears of human peripheral blood were incubated with tested substances (naphthalene, p-tretbutilfenol, 2-methyl-4-chlorophenoxyacetic acid and γ-naphthalenesulfonic acid) for 48 hours, then subjected to a differential cell count using trypan blue in Goryaev’s chamber. All the tested compounds showed no cytotoxicity in a concentration range of 1 - 100 mkM in HEK293 cells. Moreover, the ability of compounds to insignificantly inhibit the survival of mitogen-stimulated T-cells was demonstrated and more prominent effect was observed for 2-methyl-4- chlorophenoxyacetic acid.
About the Authors
L. F. Zaynullina
ФГБУН «Институт биохимии и генетики» Уфимского научного центра РАН
Russian Federation
Russian Federation
G. R. Valieva
ФГБУН «Институт биохимии и генетики» Уфимского научного центра РАН
Russian Federation
Russian Federation
G. G. Yagafarova
ФГБОУ ВО «Уфимский государственный нефтяной технический университет»
Russian Federation
Russian Federation
Yu. V. Vakhitova
ФГБУН «Институт биохимии и генетики» Уфимского научного центра РАН
Russian Federation
Russian Federation
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Review
For citations:
Zaynullina L.F., Valieva G.R., Yagafarova G.G., Vakhitova Yu.V. EVALUATION OF IMMUNO-TOXICOLOGICAL PROPERTIES OF AROMATIC COMPOUNDS. Bashkortostan Medical Journal. 2017;12(1):47-51. (In Russ.)
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