STIMULATION OF ACTOPROTECTION AFTER FORCED PHYSICAL EXERCISE AND THE USE OF ALLOGENEIC BIOMATERIAL IN THE EXPERIMENT

The main target during forced physical exercise is the muscular system. Allogeneic biomaterial (BMA) has not been previously used to correct pathological changes in muscles, and the method of its administration has not been substantiated.
The aim of the study was to determine the pathomorphological features of skeletal muscle tissue after physical exercise and the use of BMA with intramuscular, acupuncture and combined administrations.
Material and methods. Male Wistar rats (n=60) were subjected to forced swimming with a load of 10% of their body weight for 30 days. Then, in Group I (experimental), BMA suspension was administered intramuscularly. In Group II (experimental), BMA was administered using acupuncture into biologically active points. In Group III (experimental), BMA suspension was administered both intramuscularly and using acupuncture. Physiological solution was administered according to a similar scheme in control groups. After 5 and 21 days, tolerance load testing, histological examination, and morphometric analysis were performed.
Results. Long-term swimming with weights leads to overcontraction, necrosis, and fibrosis of muscle fibers. After the use of BMA, signs of rhabdomyogenesis, angiogenesis, and increased tolerance load were determined. The most pronounced effect was observed in Groups I and III.
Conclusion. BMA promotes muscle fiber restoration and early rehabilitation when administered intramuscularly.

1. Skachkov N.G., Shkurdoda V.A. Elimination and prevention of mental and physical fatigue. Terms and concepts in the field of physical culture: I International Congress. St. Petersburg. 2007: 360–361. (in Russ)

2. Todorova V., Ivanov K., Delattre C., Nalbantova V., Karcheva-Bahchevanska D., Ivanova S. Plant Adaptogens-History and Future Perspectives. Nutrients. 2021;13(8):2861. doi: 10.3390/nu13082861. (in Russ)

3. Biomaterial dlja hirurgii i sposob ego poluchenija (Biomaterial for surgery and the method of its preparation): patent № 2780831 Ros. Federacija; zajavl. 17.05.2021, opubl. 04.10.2022. Bjul. № 28. 11 s. (in Russ)

4. Karkishchenko V.N., Kapanadze G.D., Dengina S.E., Stankova N.V. Development of a method for assessing the physical endurance of small laboratory animals to study the adaptogenic activity of some drugs. Biomedicine. 2011;1(1):72-74. (in Rus)

5. Sposob stimuljacii akto-, kardio- i nejroprotekcii v uslovijah vynuzhdennoj fizicheskoj nagruzki v jeksperimente putem primenenija allogennogo biomateriala (Method of stimulation of act, cardio and neuroprotection under conditions of forced physical exertion in an experiment by using an allogeneic biomaterial): patent № 2826978 Ros. Federacija; zajavl. 14.03.2024, opubl. 19.09.2024. Bjul. № 26:15. (in Russ)

6. Kor'jak V. A., Nikolaeva L. A. Osnovy gistologicheskoj tehniki: uchebnoe posobie (Fundamentals of histological technique: a tutorial). Irkutsk: IGMU, 2020:85. (in Russ)

7. Mascorro A., Bozzola J. J. Processing Biological Tissues for Ultrastructural Study. Electron microscopy: methods and protocols. 2007. 2nd ed.:19-34. (in Engl)

8. Potolitsyna N., Parshukova O., Vakhnina N., Alisultanova N., Kalikova L., Tretyakova A., Chernykh A., Shadrina V., Duryagina A., Bojko E. Lactate thresholds and role of nitric oxide in male rats performing a test with forced swimming to exhaustion. Physiol Rep. 2023; 11(17):e15801. doi: 10.14814/phy2.15801. (in Engl)

9. Vieira C., De Lima T.C., Carobrez Ade P., Lino-de-Oliveira C. Frequency of climbing behavior as a predictor of altered motor activity in rat forced swimming test. Neurosci Lett. 2008;445(2):170-173. doi: 10.1016/j.neulet.2008.09.001. (in Engl)

10. Kawashima M, Kawanishi N, Tominaga T, Suzuki K, Miyazaki A, Nagata I, Miyoshi M, Miyakawa M, Sakuraya T, Sonomura T, Arakawa T. J Appl Physiol Icing after eccentric contraction-induced muscle damage perturbs the disappearance of necrotic muscle fibers and phenotypic dynamics of macrophages in mice. 2021; 130(5):1410-1420. doi: 10.1152/japplphysiol.01069.2020. (in Engl)

11. Nagata I., Kawashima M., Miyazaki A., Miyoshi M., Sakuraya T., Sonomura T., Oyanagi E., Yano H., Arakawa T. Icing after skeletal muscle injury with necrosis in a small fraction of myofibers limits inducible nitric oxide synthase-expressing macrophage invasion and facilitates muscle regeneration. Am J Physiol Regul Integr Comp Physiol. 2023; 324(4):R574-R588. doi: 10.1152/ajpregu.00258.2022. (in Engl)

12. Saclier M., Cuvellier S., Magnan M., Mounier R., Chazaud B. Monocyte/macrophage interactions with myogenic precursor cells during skeletal muscle regeneration. FEBS J. 2013; 280(17):4118-4130. doi: 10.1111/febs.12166. (in Engl)