THE ROLE OF THE MICROBIOTA OF THE OCULAR SURFACE AS A POSSIBLE PREDICTOR OF INFLAMMATORY DISEASES OF THE CONJUNCTIVA
Abstract
The article discusses modern approaches to the study of the microbiota of the eye surface, considering the disadvantages of traditional cultivation methods in identifying the full composition of microorganisms.
Studies show that traditional cultivation methods cannot identify all types of microorganisms, as many of them require specific conditions for growth or are uncultivated. In recent years, thanks to the use of genetic methods such as 16S rDNA sequencing, a more complete understanding of the composition of the microbiota of the ocular surface has become possible. These modern methods have made it possible to identify a variety of bacteria, such as Corynebacterium, Pseudomonas, Staphylococcus, and others, in the normal microbiota of the conjunctiva.
About the Author
R. R. Yazgarova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
References
1. Bikbov M. M., Babushkin A. E., Zainutdinova G. Kh., Matyukhina E. N. Analysis of the dynamics of some inflammatory diseases of the anterior segment of the eye in the Republic of Bashkortostan. RMZh. Klinicheskaya oftal'mologiya. 2016;16(1):34-39. (In Russ).
2. WHO launches first World report on vision [Electronic resource] URL: https://www.who.int/ru/news/item/08-10-2019-who-launchesfirst- world-report-on-vision (accessed 12.11.20024). 2019. (in Engl).
3. Kravchik M. V., Rodina E. S., Subbot A. M., Pimonova O. I., Fettser E. I., Novikov I. A. Visualization of normal ocular surface microflora via impression cytology sample using scanning electron microscopy with lanthanide contrasting. Sovremennye naukoemkie tekhnologii. 2022;138(6):5-13. (In Russ) DOI 10.17116/oftalma20221380615.
4. Lebedev O.I., Surov A.V., Akent'eva E.V. Features of ophthalmic inflammatory diseases results of a federal study. Oftal'mologicheskie vedomosti. 2014;7(2):13-17. (In Russ).
5. Maichuk Yu.F. Glaznye infektsii (Eye infections) RMZh. 1999;(1):1-7. (In Russ).
6. Okolov I.N., Gurchenok P.A., Vokhmyakov A.V. Normal conjunctival microflora in patients undergoing ophthalmic surgery. Oftal'mologicheskie vedomosti. 2008;1(3):18-21. (In Russ).
7. Pal'tsev M. A., Belushkina N. N., Chaban E. A. 4p-medicine as a new model of healthcare in the Russian Federation. Orgzdrav. Novosti, mneniya, obuchenie. 2015;(2):48-54. (In Russ).
8. Rezbaeva G.N., Orenburkina O.I., Gimranova I.A., Babushkin A.E., Gazizullina G.R. The normal microbiota of the ocular surface and the connection between the changes in its composition and ophthalmic pathologies. Rossiiskii oftal'mologicheskii zhurnal. 2024;17(1);144-148. (In Russ) DOI 10.21516/2072-0076-2024-17-1-144-148.
9. Sokolenko A.V. Uncultivated forms of bacteria: spread in nature, inductors of uncultivated condition and backward mutation. Sovremennye naukoemkie tekhnologii. 2006;(2):11-15. (In Russ).
10. Bharathi M.J., Ramakrishnan R., Meenakshi R., Padmavathy S., Shivakumar C., Srinivasan M. Microbial keratitis in South India: influence of risk factors, climate, and geographical variation. Ophthalmic Epidemiol. 2007;14(2):61-69. (in Engl) DOI: 10.1080/09286580601001347.
11. Butcher R.M.R., Sokana O., Jack K., Kalae E., Sui L., Russell C., Houghton J., Palmer C., Holland M. J., Le Mesurier R. T., Solomon A. W., Mabey D. C. W., Roberts C. H. Active Trachoma Cases in the Solomon Islands Have Varied Polymicrobial Community Structures but Do Not Associate with Individual Non-Chlamydial Pathogens of the Eye. Frontiers in Medicine. 2018;4:251. (in Engl) DOI: 10.3389/fmed.2017.00251.
12. Cavuoto K. M., Banerjee S., Miller D., Galor A. Effect of clinical parameters on the ocular surface microbiome in children and adults. Clinical Ophthalmology. 2018;2;12:1189-1197. (in Engl) DOI: 10.2147/OPTH.S166547.
13. Cavuoto K.M., Banerjee S., Sharma S., Galor A. Composition and Comparison of the Ocular Surface Microbiome in Infants and Older Children. Translational Vision Science & Technology. 2018;7(6):16. (in Engl) DOI: 10.1167/tvst.7.6.16.
14. Chen Z., Xiang Z., Cui L., Qin X., Chen S., Jin H., Zou H. Significantly different results in the ocular surface microbiome detected by tear paper and conjunctival swab. BMC Microbiology. 2023;23(1):31. (in Engl) DOI: 10.1186/s12866-023-02775-3.
15. Delbeke H., Younas, S., Casteels, I., Joossens, M. Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data. Acta Ophthalmologica. 2021;99(1):16-25. (in Engl) DOI: 10.1111/aos.14508.
16. Doan T., Akileswaran L., Andersen D., Johnson B., Ko N., Shrestha A., Shestopalov V., Lee C. S., Lee A. Y., Van Gelder R. N. Paucibacterial Microbiome and Resident DNA Virome of the Healthy Conjunctiva. Investigative Ophthalmology & Visual Science. 2016;57(13):5116-5126. (in Engl) DOI: 10.1167/iovs.16-19803. PMID: 27699405; PMCID: PMC5054734.
17. Dong Q., Brulc J. M., Iovieno A., Bates B., Garoutte A., Miller D., Revanna K. V., Gao X., Antonopoulos D. A., Slepak V. Z., Shestopalov V. I. Diversity of bacteria at healthy human conjunctiva. Investigative Ophthalmology & Visual Science. 2011;52(8):5408-5413. (in Engl) DOI: 10.1167/iovs.10-6939. PMID: 21571682; PMCID: PMC3176057.
18. Dong X, Wang Y, Wang W, Lin P, Huang Y. Composition and Diversity of Bacterial Community on the Ocular Surface of Patients With Meibomian Gland Dysfunction. Investigative Ophthalmology & Visual Science. 2019;60(14):4774-4783. DOI: 10.1167/iovs.19-27719. Erratum in: Invest Ophthalmol Vis Sci. 2020;61(13):26. (in Engl) DOI: 10.1167/iovs.61.13.26.
19. Graham J. E., Moore J. E., Jiru X., Moore J. E., Goodall E. A., Dooley J. S., Hayes V. E., Dartt D. A., Downes C. S., Moore T. C. Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes. Investigative Ophthalmology & Visual Science. 2007;48(12):5616-23. (in Engl) DOI: 10.1167/iovs.07-0588.
20. Ham B., Hwang H. B., Jung S. H., Chang S., Kang K. D., Kwon M. J. Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects. Current Eye Research. 2018;43(3):314-324. (in Engl) DOI: 10.1080/02713683.2017.1406528.
21. Huang Y. S., Dai Y. H., Sun S. Y., Lan J., Xie L. X. Study of different methods in reducing conjunctival bacteria before cataract surgery. Zhonghua Yi Xue Za Zhi. 2009;89(35):2458-61. (In Chinese)
22. Huang Y., Yang B., Li W. Defining the normal core microbiome of conjunctival microbial communities. Clinical Microbiology and Infection. 2016;22(7):643-643. (in Engl) DOI: 10.1016/j.cmi.2016.04.008. Epub 2016 Apr 19. PMID: 27102141.
23. Li S., Yi G., Peng H., Li Z., Chen S., Zhong H., Chen Y., Wang Z., Deng Q., Fu M. How Ocular Surface Microbiota Debuts in Type 2 Diabetes Mellitus. Front Cell Infect Microbiol. 2019;1-9. (in Engl) DOI: 10.3389/fcimb.2019.00202.
24. Lee S.H., Oh D.H., Jung J.Y., Kim J.C., Jeon C.O. Comparative ocular microbial communities in humans with and without blepharitis. Invest Ophthalmol Vis Sci. 2012;53(9):5585-93. (in Engl) DOI: 10.1167/iovs.12-9922.
25. Ozkan J., Nielsen S., Diez-Vives C., Coroneo M., Thomas T., Willcox M. Temporal Stability and Composition of the Ocular Surface Microbiome. Scientific Reports. 2017;7(1):9880. (in Engl) DOI: 10.1038/s41598-017-10494-9.
26. Shin H., Price K., Albert L., Dodick J., Park L., Dominguez-Bello M. G. Changes in the Eye Microbiota Associated with Contact Lens Wearing. mBio. 2016;7(2):e00198. (in Engl) DOI: 10.1128/mBio.00198-16.
27. Willcox M. D. Characterization of the normal microbiota of the ocular surface. Experimental Eye Research. 2013;117:99-105. (in Engl) DOI: 10.1016/j.exer.2013.06.003. Epub 2013 Jun 22.
28. Zhou Y., Holland M. J., Makalo P., Joof H., Roberts C. H., Mabey D. C., Bailey R. L., Burton M. J., Weinstock G. M., Burr S. E. The conjunctival microbiome in health and trachomatous disease: a case control study. Genome Med. 2014;6(11):99. DOI: 10.1186/s13073- 014-0099-x. (in Engl)
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For citations:
Yazgarova R.R. THE ROLE OF THE MICROBIOTA OF THE OCULAR SURFACE AS A POSSIBLE PREDICTOR OF INFLAMMATORY DISEASES OF THE CONJUNCTIVA. Bashkortostan Medical Journal. 2024;19(6):105-110. (In Russ.)
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