FEATURES OF ARCHITECTONICS OF THE NEWLY FORMED VASCULAR SYSTEM IN DIABETIC RETINOPATHY

Abstract

The objective of the work was to study the features of the architectonics of newly formed vessels in proliferative diabetic retinopathy (PDR).67 epiretinal membranes removed during vitrectomy in patients with PDR, were studied by immunofluorescence and light microscopy. Antibodies to the following markers were used: von Willebrand factor (vWF) (Chemicon, USA), GAFP, ribonucleotide reductase (RR), (Umea, Sweden), DM 34, DM 45, Type IV collagen; then they were stained with secondary antibodies.135 light images of epiretinal membranes were studied by light microscopy. Before and after vitrectomy, in all patients who had received material for pathomorphological examination we performed color and black-and-white photos of the fundus and autofluorescence.Removed by a single block, the epiretinal membrane with PDR was a total film preparation in which all the structural elementsof the posterior membrane and the newly formed tissue were well visualized. The density of newly formed capillaries in the advanced stages of PDR increased at a distance from the epicenter of growth, and their caliber progressively decreased. The trunk vessels anastomosed with each other only in the upper (distal) sections. The architectonics of the newly formed vasculature with PDR varied, but the main feature was the absence of the signs of trophic function of such a hemocirculation system.