THE DENSITOMETRIC QUANTITATION OF SOME DRUGS IN WHOLE BLOOD

Abstract

The study objective was the development of quantitation technique for some drugs in whole blood using thin-layer chromatography with videodensitometry. The study subject was model samples of whole blood containing morphine, codeine, verapamil, baclofen, doxylamine, amitriptyline. The identification parameters of drugs by thin-layer chromatography were defined, interferences of blood matrix and other drugs were studied. Limits of detection in blood samples were 600,0 ng/ml (5,0 μg/spot) for morphine, codeine, amitriptyline, 150,0 ng/ml (1,0 μg/spot) for verapamil, doxylamine, 75,0 ng/ml (0,5 μg/spot) for baclofen. For drug quantitation the chromatograms were scanned, images were processed and calibration models were designed using videodensitometry computer program. The calibration model is described by polynomial (square) regression. The limits of quantitation for different drugs were from 300,0 to 1500,0 ng/ml; accuracy and precision does not exceed 25%.Thus, a quantitation technique for analysis of some drugs in whole blood by thin-layer chromatography with videodensitometry was developed. The ranges of quantitation allow using this technique for forensic chemistry and clinical analytical toxicology.