HISTOLOGICAL FEATURES OF THE CONJUNCTIVA AND THE SCLERA OF THE EYEBALL AT INTRAOPERATIVE APPLICATION OF 0.05% SOLUTION OF CYCLOSPORINE A

Abstract

Object is the analysis of histological changes of conjunctiva and sclera regeneration after surgical intervention with intraoperative application of 0,05% solution of Cyclosporine A.The study was conducted on Wistar rats (n = 48), they were divided into 2 groups: the main (n = 32), including subgroups "a" (n= 16) and "b" (n = 16) and the comparison group (n = 16). We performed a through cut of the conjunctiva and the surface layers of the sclera. A hemostatic sponge impregnated with 0,05% Cyclosporine A solution was applied to the surgical intervention zone. In the subgroup "a" duration of application was 3 minutes, in the subgroup "b" - 6 minutes. In the comparison group application of the hemostatic sponge without a cytostatic was performed.A six-minute application of 0,05% Cyclosporine A solution was found to cause a lesser degree of infiltrative changes, which is caused by the suppression of cell migration to the surgical intervention zone. In contrast, early development of fibrosis changes was noted in the comparison group, where application of cytostatic was not performed.Using a 0,05% solution of Cyclosporine A in the form of intraoperative application to the zone of a surgical trauma causes slowing of regenerative processes. The greatest effectiveness is observed in case of 6 minutes application.