DEVELOPMENT OF THE METHOD FOR QUANTITATIVE DETERMINATION OF FLAVONOIDS IN HEPATOPROTECTIVE COLLECTION

Abstract

In connection with a steady increase in the number of liver diseases, the search for new herbal formulations that have a hepatoprotective effect is topical. To introduce new medicinal herbal preparations into practice, it is necessary to develop a regulatory document regulating the quality of herbal formulations. The article deals with the development of a methodology for the quantitative determination of flavonoids in a new hepatoprotective preparation. To develop the technique, a spectrophotometric method was used. Various parameters of extraction of flavonoids have been studied. Optimal conditions for the extraction of flavonoids (raw materials, ethyl alcohol concentration, extraction time), the amount of complexing agent, and the formation of a complex of flavonoids with aluminum chloride were detected. According to the research, a spectrophotometric method for quantifying the amount of flavonoids in terms of hyperoside was developed. The content of flavonoids in the hepatoprotective collection averaged 1.53±0.03%. Based on the data obtained, a numerical indicator was developed for the inclusion in the draft of a pharmacopoeial article for hepatoprotective collection - the content of flavonoids is not less than 1.2%.