EXPERIMENTAL RESEARCH OF THE ISCHEMIA OF ABDOMINAL CAVITY AND RETROPERITONEUM ORGANS IN CASE OF INTRA-ABDOMINAL HYPERTENSION ASSOCIATED WITH ACUTE BLOOD LOSS

The aim of the research was to study the impact of intraperitoneal hypertension and acute blood loss on the severity of ischemia of abdominal cavity and retroperitoneal organs. The research was conducted on 36 meat piglets of both sexes with an average weight of 11,7±0,7 kg for the purpose of studying the blood lactate indices as a marker of tissue ischemia as well as living organs ischemia at the levels of the intra-abdominal pressure modelled in an experiment as 15, 20 and 25 mmHg on a background of the modelled acute loss of 10 and 20% of blood volume. Under intratracheal anesthesia and through the catheterized arterial vein the required volume of the circulating blood was collected within 30 minutes. Intraperitoneal hypertension was modelled by creating an extended pneumoperitoneum with the help of an insufflator. The blood lactate index was measured before the intense pneumoperitoneum and during it at the 180th minute. The structure of these data allowed us to use the method of variance analysis. Since we dealt with the results of accurate measurements it was possible to use a very informative method - Fisher’s parametric analysis of variance. As the groups volume turned out to be small for the reliability of statistical evaluations Kruskal-Wallis one-way analysis of variance was used. For the pairwise intergroup comparison Mann-Whitney U-test was applied.Outside the intraperitoneal hypertension the blood lactate index in the tested animals was 2,29±0,32 mmol/L. In regard to the average index of lactate before the intraperitoneal hypertension the animals of all the groups were in equal «starting points». On the background of acute blood loss and the increase of intraperitoneal pressure the blood lactate index rises that testifies the develop-ment of intraperitoneal ischemia. And with the increasing volume of the lost blood at the same indices of intraperitoneal pressure thelactate index also increases significantly, that means a direct positive correlation of the severity of the abdominal cavity ischemia and retroperitoneal space with the severity of blood loss under the intra-abdominal hypertension.

внутрибрюшное давление, внутрибрюшная гипертензия, компартмент-синдром, острая кровопотеря, ишемия, intraperitoneal pressure, intraperitoneal hypertension, compartment syndrome, acute blood loss, ischemia