DEVELOPMENT OF METHODS FOR QUANTITATIVE DETERMINATION OF POLYSACCHARIDES OF BIRCH LEAVES

Abstract

Natural polysaccharides, possessing a wide spectrum of physiological effects, can be promising drug candidates. The methods proposed by the State Pharmacopoeia for the determination of sugars are not always suitable for the analysis of complex polymers. The purpose of this article is modification of the anthrone-sulfur method for the quantitative determination of birch leaves polysaccharides ( Betula pendula Roth., Betula pubescens Ehrh. ) in a substance. The hydrolysis parameters were tested: it was proposed to carry out hydrolysis for 30 minutes in a boiling water bath with 98% sulfuric acid. It is proposed to replace the standard substance by rhamnose, due to the nature of the composition of the object being studied. Ultrafiltration should be applied before analysis to increase the specificity of the method. It was established that the method is valid by all studied characteristics.