EVALUATION OF THE METHOD OF HUMAN SPERM CELL DEATH IN VITRO

Abstract

The aim of the study was to assess the methods of death of human ejaculatory sperm by methods of apoptosis and necrosis in vitro.The ejaculates of 40 fertile men aged 22 to 38 years were studied. Sperms were isolated from ejaculate samples, washed fromthe seminal plasma with phosphate-salt buffer. Motility and viability of germ cells, including in the presence of apoptosis marker (externalization of phosphatidylserine, detected by binding to Annexin-V) were investigated in freshly isolated sperm and sperm after 4 and 24 hours of incubation in vitro at 37°C.After 4 hours of incubation in vitro no significant changes in sperm parameters were found, but after 24 hours of incubation there was a decrease in motility and viability of germ cells. However, these changes were not accompanied by any increase in the level of gametes with the marker of apoptotic process. The study found that ejaculatory sperm of a healthy person does not trigger the process of apoptosis at least in vitro.