ANTIBIOTIC RESISTANCE OF CLINICAL STRAINS OF HAFNIA ALVEI

The aim of the study was to determine the spectrum of drug resistance of Hafnia alvei clinical strains to the most widespread antibacterial drugs. The work evaluated sensitivity of bacteriophages to Hafnia alvei strains. Antibiotic resistance was established by disk diffusion test, where disks were saturated with 23 widely used antibiotics: penicillin, ampicillin, ampicillin/sulbactam, oxacillin, carbenecillin, lincomycin, kanamycin, erythromycin, rifampicin, streptomycin, gentamycin, tobramycin, norfloxacin, gemifloxacin, moxifloxacin, tetracycline, axetine cefuroxime, ceftazidime, cefepime, chloromycetin, nystatin, and with nitrofurans: nitrofurantoin, furaginum on 80 Hafnia alvei clinical strains, taken from patients with gastro-intestinal, urological and pyoinflammatory diseases. Clinical material was obtained from bacteriological laboratories of the Republic of Bashkortostan. Bacteriophage was received by a common method from laboratory clinical wastes. To determine sensitivity of bacteriophage to Hafnia alvei clinical strains spot-test was applied. Analysis of the results showed that among Hafnia alvei clinical strains 7.5% (6 units) were resistant to cefepime, 11% (9 units) to ceftazidime, 15% (12 units) - to gemifloxacin, 13.7% (11 units) - to moxifloxacin and were equally resistant to ampicillin, ampicillin/sulbactam, axetine cefuroxime, cefolatin - 90±3.75. Bacteriophage can lyze 65 cultures (81%) from80 Hafnia alvei clinical strains, including antibiotic resistant - 60 strains (75%). Summering the results, one may conclude, that ceftazidime, cefepime, gemifloxacin, moxifloxacin and bacteriophage can be used as treatment and preventive drugs during antibacterial therapy of infections caused by Hafnia alvei bacteria, including antibiotic resistant strains.

бактерии Hafnia alvei, метод «бумажных дисков», антибиотики, ампициллин, ампициллин/сульбактам, цефуроксимаксетин, антибиотикорезистентность, бактериофаги, Hafnia alvei bacteria, disk diffusion test, antibiotics, ampicillin, ampicillin/sulbactam, axetine cefuroxime, antibiotic resistance, bacteriophages