ANTAGONISTIC ACTIVITY OF A PROBIOTIC FROM SPORE-FORMING BACTERIA OF THE GENUS BACILLUS RELATINGTO MICROFLORA, SEPARATED FROM PERIODONTAL POCKET CONTENTS

Abstract

The antagonistic activity of the probiotic Bacillus subtilis 3H was studied with respect to 4 reference strains of yeast-like fungi of the genus Candida (C. albicans 001, C. albicans No. STC885-653, C. stellatoidea ATCC 10264, C. Krusei Harv ATCC 6259), 6 clinical isolates (C. albic, C. Krusei, C. stellatoidea, C. tropicalis, C.glabrata, C. guilliermondi), as well as testing cultures (Staphilococcus aureus, Escherichia coli, Streptococcus mitis, Streptococcus mutans, Streptococcus sanguis, Streptococcus agalactis) and clinical strains of periodontopathogenic microflora (Staphilococcus aureus, Streptococcus mitis, Streptococcus mutans, Streptococcus sanguis, Streptococcus agalactis, Streptococcus salivarius, Prevotella intermedia, Treponema denticola, Agregatibacter actinomycetecomitans), separated from periodontal pocket contents. Minimal suppressive concentration was performed by diffusion method (perpendicular streak technique).The results of the study show that all 4 referent strains of fungi of Candida genus are sensitive Bacillus subtilis 3H probiotic. C. Albicans and C. Krusei are more sensitive than the rest 4 clinical isolates. When inhibition zones of periodontopathogenic microorganisms were compared, the smallest zone was identified in Streptococcus mitis, and the largest in Streptococcus agalactis and Staphilococcus aurius.