PHENOL COMPOUNDS FROM JUNIPERUS COMMUNIS L. VAR. SAXATILIS
Abstract
The genus Juniperus L. is the largest in the Cupressaceae family, the species of which are widely used in folk medicine of different countries.The goal is to determine the quantitative and qualitative content of phenolic compounds in Juniperus communis growing in Yakutia.Material and methods: The needles of J. communis, collected in the Verkhoyansk district of the Republic of Sakha (Yakutia) in2019, were the object of the study. The total content of phenolic compounds was determined by the Folin-Ciocaltheu method. The qualitative and quantitative composition of phenolic compounds was studied using HPLC-UV.Results: As a result of metabolic analysis, the presence of 17 different compounds in the needles of J. communis was identified,including phenylpropanoids and their glycosides, flavonoids both in the form of glycoside and in the form of aglycon, catechin, procyanidins and terpenes. The dominant compounds in J. communis are ellagic acid (14,97 ± 0,31 mg / g), catechin (5,71 ± 0,11 mg / g) and rutin (3,54 ± 0,07 mg / g).Conclusions: Alcohol extraction of needles of J. communis contains up to 37,7% ± 0,5 phenolic compounds. The results prove the promise of the use of needles J.communis as a medicinal plant material.
About the Authors
V. V. Chemposov
ФГАОУ ВО «Северо-Восточный федеральный университет имени М.К. Аммосова»
Russian Federation
Russian Federation
A. G. Vasilieva
ФГАОУ ВО «Северо-Восточный федеральный университет имени М.К. Аммосова»
Russian Federation
Russian Federation
N. K. Chirikova
ФГАОУ ВО «Северо-Восточный федеральный университет имени М.К. Аммосова»
Russian Federation
Russian Federation
References
1. Князева С.Г. Внутрипопуляционная изменчивость можжевельника обыкновенного / С.Г. Князева// Хвойные бореальной зоны. - 2010. - №1-2. - C. 91-96
2. Атлас лекарственных растений Якутии / Л.В. Кузнецов [и др.] - Якутск: Изд-во СО РАН, 2003 - 194 с
3. Adams R. P. Junipers of the World: The genus Juniperus / R. P. Adams / 4th Ed. Trafford Publishing Co., Bloomington, IN. - 2014. - 417 p
4. Государственная фармакопея Российской Федерации / МЗ РФ. - XIII изд. - М., 2015. - 1470 с
5. Кароматов, И.Д. Можжевельник в народной и научной медицине / И.Д. Кароматов, М.С. Давлатова // Биология и интегративная медицина. - 2018. - №1. URL: https://cyberleninka.ru/article/n/mozhzhevelnik-v-narodnoy-i-nauchnoy-meditsine/viewer (дата обращения: 16.11.2020)
6. Макаров, A.A. Растительные лечебные средства якутской народной медицины / А.А. Макаров. - Якутск: Якут. кн. изд-во, 1974. - С. 42-43
7. Макаров, А.А. Лекарственные растения Якутии и перспективы их освоения / А.А. Макаров. - Новосибирск: Изд-во Сибирского отделения РАН, 2002. - 264 c
8. Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills / I. Fierascu [et al]. // BMC complementary and alternative medicine. - 2018. - Т. 18. - №. 1. - 3 p
9. Ložienė K. Juniper (Juniperus communis L.) Oils / K. Ložienė, P.R. Venskutonis // Essential Oils in Food Preservation, Flavor and Safety. - 2016
10. Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis / B. Vasilijević [et al] // Food and chemical toxicology. - 2018. - Т. 112. - P. 118-125
11. Raasmaja A. The Water Extract of Juniperus communis L. Induces Cell Death and Sensitizes Cancer Cells to Cytostatic Drugs through p53 and PI3K/Akt Pathways / A. Raasmaja, U. Stenius, A. Ghalali // International journal of molecular sciences. - 2019. - Т. 20. - №. 9. - 2054 p
12. Кожанова, К.К. Фитохимические исследования СО2 экстракта из плодов можжевельника обыкновенного (Juniperus communis) / К.К. Кожанова, Г.Ғ. Полатова // Вестник КазНМУ. - 2018. - №2. - С. 269-271
13. Зырянова, Ю.В. Химический состав можжевельника сибирского, каллусной ткани и послеэкстракционного остатка / Ю.В. Зырянова, Е.Н. Аёшина, Н.А. Величко // Химия растительного сырья. - 2012. - №2. - С. 145-150
14. Компонентный состав и антибактериальная активность эфирного масла древесной зелени juniperus communis l. субарктической зоны России / Н.А. Самсонова [и др.] // Сибирский лесной журнал. 2020. - №2. - С. 31-39
15. Ved A. Antioxidant and hepatoprotective potential of phenol-rich fraction of Juniperus communis Linn. leaves / A. Ved, A. Gupta, A. K. S. Rawat // Pharmacognosy magazine. - 2017. - Т. 13. - №. 49. - 108 p
16. Polyphenolic profile and targeted bioactivity of methanolic extracts from Mediterranean ethnomedicinal plants on human cancer cell lines / Pollio A. [et al.] // Molecules. - 2016. - Т. 21. - №. 4. - 395 p
17. Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis / Vasilijević B. [et al] // Food and chemical toxicology. - 2018. - Т. 112. - P. 118-125
18. Baerheim Svendsen A. A comparative study of the composition of the essential needle oils of Norwegian low lands Juniper and high mountains Juniper / A. Baerheim Svendsen, J. J. C. Scheffer, A. Looman // Scientia Pharmaceutica. - 1985. - Т. 53. - P. 159-161
19. Kallio H. Maritime influence on the volatile terpenes in the berries of different ecotypes of juniper (Juniperus communis L.) in Finland / H. Kallio, K. Junger-Mannermaa // Journal of Agricultural and Food Chemistry. - 1989. - Т. 37. - №. 4. - P. 1013-1016
20. Effect of latitude and altitude on the terpenoid and soluble phenolic composition of juniper (Juniperus communis) needles and evaluation of their antibacterial activity in the boreal zone / Martz F. [et al.] // Journal of agricultural and food chemistry. - 2009. - Т. 57. - №. 20. - P. 9575-9584
21. Singleton V.L. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. / V.L. Singleton, R. Orthofer, R.M. Lamuela-Raventos // Methods in Enzymology. - 1999. - Vol.299. - Р. 152-178
22. Polyphenolic compounds from the leaves of Juniperus oxycedrus L. subsp. macrocarpa (Sm.) Ball. / Stassi V. [et. al.] // Pharmaceutica Acta Helvetiae. - 1998. - Т. 72. - №. 5. - P. 311-312
23. Yaglioglu A. S. Screening of some Juniperus extracts for the phenolic compounds and their antiproliferative activities / A. S. Yaglioglu, F. Eser // South African Journal of Botany. - 2017. - Т. 113. - P. 29-33
24. Sakar M. K. Flavonoide in Blättern von Juniperus drupacea / M. K. Sakar, H. Friedrich // Planta medica. - 1984. - Т. 50. - №. 01. - P. 108-109
25. Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species / A. K. Maurya [et al] // Chemistry & biodiversity. - 2018. - Т. 15. - №. 9. - P. e1800183
26. Antifungal and repellent activities of the essential oils from three aromatic herbs from western Himalaya / I. Stappen [et al.] // Open Chemistry. - 2018. - Т. 16. - №. 1. - P. 306-316
27. Antibacterial and biofilm inhibitory activity of medicinal plant essential oils against Escherichia coli isolated from UTI patients / R. Lagha [et al.] // Molecules. - 2019. - Т. 24. - №. 6. - 1161 p
28. A colorimetric broth microdilution method for assessment of Helicobacter pylori sensitivity to antimicrobial agents / P. Knezevic [et al.] // Journal of pharmaceutical and biomedical analysis. - 2018. - Т. 152. - P. 271-278
Review
For citations:
Chemposov V.V., Vasilieva A.G., Chirikova N.K. PHENOL COMPOUNDS FROM JUNIPERUS COMMUNIS L. VAR. SAXATILIS. Bashkortostan Medical Journal. 2020;15(5):72-76. (In Russ.)
Views:
69
Email this article 
