VALIDATION OF THE METHODOLOGY FOR THE QUANTITATIVE DETERMINATION OF FLAVONOIDS IN A HERBAL COLLECTION WITH HYPOGLYCEMIC ACTIVITY
Abstract
Validation of pharmacopoeial methods is carried out at the stage of development of normative documentation for new medicines. It’s aim is to confirm the validity of the method for determining the indicators and quality standards of pharmaceutical products.The aim of the research was to validate the developed methodology for the quantitative determination of luteolin in plant composition with hypoglycemic activity.Material and methods: the object of the study was plant composition with hypoglycemic activity developed at the Departmentof pharmacognosy with the course of botany and fundamentals of phytotherapy of the BSMU. Quantitative determination was carried out by the spectrophotometric method using a complexing component. We selected optimal conditions for the method of spectrophotometric quantification of luteolin, and then the proposed method was validated.The results of the studies showed the linearity, repeatability and precision of the developed method of spectrophotometric quantification of luteolin.Conclusions: the developed method can be used to standardize the plant composition with hypoglycemic activity.
About the Authors
A. R. Ahmet’Yanova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
R. R. Shakirova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
N. V. Kudashkina
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Ahmet’Yanova A.R., Shakirova R.R., Kudashkina N.V. VALIDATION OF THE METHODOLOGY FOR THE QUANTITATIVE DETERMINATION OF FLAVONOIDS IN A HERBAL COLLECTION WITH HYPOGLYCEMIC ACTIVITY. Bashkortostan Medical Journal. 2020;15(6):56-61. (In Russ.)
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