HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY IN THE ANALYSIS OF TRITERPENE SAPONINS GYNOSTEMMA PENTAPHYLLUM

Objective: to study the triterpene saponins of the herb Gynostemma pentaphyllum, introduced in the Republic of Bashkortostan, by the method of high-performance thin-layer chromatography.Material and methods: the object of the study was the herb of Gynostemma pentaphyllum, which is a dried aerial part: stemswith simple long-petiolate deeply palmately dissected leaves and small flowers in paniculate or racemose inflorescences. The studies were carried out by the method of high-performance thin-layer chromatography (HPTLC), which is based on obtaining chromatograms on a thin layer of sorbent with subsequent recording of «fingerprints» in the software. Results and conclusions: in the course of laboratory studies, optimal conditions were selected for conducting chromatographic analysis of alcoholic extracts of the herb. The results of a qualitative analysis of the gynostemma pentaphyllum raw materials by the HPTLC method showed the presence of triterpene saponins, of which ginsenosides and β-escin were identified, the latter was first established in the introduced raw material. It was also found that the dominant triterpene saponin in the analyzed samples was β-escin (Rf = 0,45), which has a dark orange fluorescence in ultraviolet light after staining with a specific reagent. The quantitative content of the dominant saponin in the herb of Gynostemma pentaphyllum was 0,317±0,011%.

herb Gynostemma pentaphyllum, chromatodensitometry, triterpene saponins, fingerprints, β-escin, ginsenosides, hypenosides

1. Государственная фармакопея Российской Федерации XIV изд. [Электронный ресурс] // Федеральная электронная медицинская библиотека. 2018. URL: http://www.femb.ru (дата обращения: 10.12.20)

2. Еремин, В.М. Красная книга Сахалинской области: растения и грибы / В. М. Еремин. - Кемерово, 2019. - 354 с

3. Хасанова, С.Р. Возможности использования денситометрии в стандартизации лекарственного растительного сырья / С.Р. Хасанова, Н.В. Кудашкина, Р.Р. Файзуллина // Человек и лекарство: сборник матер. ХХI Российского национального конгресса. - 2014. - С. 347-348

4. Anti-cancer effects of Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan) / Y. Li // Journal Chinese Medicine. -2016. Vol. 11, № 1. - P. 43-58

5. Chromatographic and spectral fingerprinting standartization of traditional medicines: an overview as modern tools / L. Giri [et al.] // Research Journal of Phitochemistry. - 2010. - Vol. 4, № 4. - P. 234

6. Hariprasad, P. Chromatographic fingerprint analysis of Rumex vesicarius L. by HPTLC technique/ P. Hariprasad, N. Ramakrishnan // Asian Pacific Journal of Tropical Biomedicine. - 2012. - № 1. - P. 57

7. Mishra, R.N. Jiao Gu Lan (Gynostemma pentaphyllum): The Chinese rasayan-current research scenario / R.N. Mishra, D. Joshi //j. Res Pharm Biomed Sci. - 2011. - Vol. 4, № 2. - P. 1483-1502

8. Navratilova, Z. Gynostemma pentaphyllum - active compounds and therapeutic effects / Z. Navratilova. //j. Prakticke lekarenstvi. - 2017. Vol. 13, № 3. - P. 116-118

9. Nikam, P.H. Future trends in standardization of herbal drugs / P. H. Nikam, J. Kareparamban, A. Jadhav, V. Kadam. // Journal of Applied Pharmaceutical Science. - 2012. - Vol. 02, № 06. - P. 38