SEASONAL CHANGES IN THE ANTIOXIDANT ACTIVITY AND THE CONTENT OF THE MAIN GROUPS OF SUBSTANCES IN COMMON SPRUCE CONES
Abstract
Objective. The research of the antioxidant activity of the extract of the cones of the common spruce obtained from raw materials of different harvesting periods.
Material and methods. The object of the study are common spruce cones harvested on the territory of the Ilyinsky district of Perm Krai from July 2020 to March 2021. Extracts were obtained according to the procedure specified in the patent (№2756009 of 24.09.2021) and the methodology of the State Pharmacopoeia of the 14th edition. To determine the antioxidant activity, a reaction with a stable free radical 2,2-diphenyl-1-picrylhydrazyl was used. The content of flavonoids was determined spectrophotometrically according to the method developed by us.
Results and discussion. It was found that the greatest antioxidant activity is observed in extracts obtained from raw materials harvested in the period from September (IC50 = 28.02 ±0.68) to December (IC50 = 32.68±2.29). The antioxidant activity of the ex- tract obtained by the method specified in the RU №2756009 patent is significantly higher than the antioxidant activity of the extract obtained by the pharmacopoeia method. The maximum accumulation of flavonoids in the cones of spruce is observed in January (0.22 ± 0.04%). Since August (37.92±5.89%), there has been an increase in the content of oxidizable substances.
Conclusions. The results obtained show the need to extend the harvesting time of the common spruce cones to get extracts with the maximum content of active substances and the greatest antioxidant activity.
About the Authors
D. O. Semakin
ФГБОУ ВО «Пермская государственная фармацевтическая академия» Минздрава России
Russian Federation
Russian Federation
D. K. Gulyaev
ФГБОУ ВО «Пермская государственная фармацевтическая академия» Минздрава России
Russian Federation
Russian Federation
V. D. Belonogova
ФГБОУ ВО «Пермская государственная фармацевтическая академия» Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Semakin D.O., Gulyaev D.K., Belonogova V.D. SEASONAL CHANGES IN THE ANTIOXIDANT ACTIVITY AND THE CONTENT OF THE MAIN GROUPS OF SUBSTANCES IN COMMON SPRUCE CONES. Bashkortostan Medical Journal. 2022;17(6):37-41. (In Russ.)
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