MIGRATION CELL FOR EXPERIMENTAL STUDY OF TUMOR CELL ACTIVITY

Abstract

The most important property of living cells is the ability to move or migrate. The study of migration in an environment simulat-ing in vivo conditions using 3D microfluidic devices will lay the foundation for the development of a miniature personalized diag-nostic device for oncological diseases.
The purpose of the research is to study the active movement of cells in a developed and manufactured two-level migration unit.
Material and methods. Standard modern methods for cultivating prostate cancer cells PC-3 were used. Microchannels were fab-ricated by soft photolithography. Measurement of the hydrodynamic characteristics of the flow was carried out using optical micros-copy methods based on the analysis of frame-by-frame images of a changing flow.
Results. To study the active movement of cells by soft photolithography, a two-level migration unit was developed and manu-factured. The ability of PC-3 cancer cells to migrate in the developed unit was experimentally demonstrated in the present article, the migration rates of PC-3 cells in migration channels 15 and 25 μm wide were calculated. The average migration rate in a channel 15 μm wide was 0.45 μm/min, 25 μm - 0.85 μm/min.
Conclusions. The uniqueness of the results obtained is due to the use of the developed new method allowing to study not only the migration characteristics of cells, but also their deformation which affects movement in space-constrained conditions. The de-velopment of a relatively simple 3D device for monitoring the migration of tumor cells will undoubtedly be an important step to-wards improving the diagnosis and treatment of cancer.