MORPHOMETRIC STUDY OF THE HEAVY METALS' EFFECTS ON THE RENAL CORTEX OF PREPUBERTAL RATS
Abstract
Purpose – morphometric study of the effects of Cd and Pb heavy metals on the renal cortex of Wistar rats of prepubertal age.
Material and methods. During 3 weeks male Wistar rats (adolescents, aged 4 weeks) per os received Cd sulfate solution at a dose of 0.5 mg/kg (group 2), Pb acetate solution at a dose of 10 mg/kg (group 3), or their combination (group 4). The control group (group 1) received saline. Morphometric study of renal corpuscles, proximal and distal tubules on semi-thin sections of the renal cortex was performed.
Results and discussion. Under conditions of chronic exposure to subtoxic doses of Cd and/or Pb, statistically significant histological changes were revealed comparing to the control group: an increase in the area of the free Bowman’s spaces and the total area of the renal corpuscle; decrease in the height of epithelial cells of the proximal and, to a lesser extent, distal tubules, with an increase in the diameter and area of the tubular lumen. The most prominent histological changes were induced by Pb used singly.
Conclusions. Using morphometry, the prerequisites for the development of structural and functional disorders of the kidneys were revealed, such as atrophy of the epithelium of the proximal tubules and congestive changes in the renal corpuscles.
About the Authors
P. A. Elyasin
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
S. V. Zalavina
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
A. N. Mashak
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
R. B. Galenok
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
G. V. Pravotorov
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
S. V. Aidagulova
ФГБОУ ВО «Новосибирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
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For citations:
Elyasin P.A., Zalavina S.V., Mashak A.N., Galenok R.B., Pravotorov G.V., Aidagulova S.V. MORPHOMETRIC STUDY OF THE HEAVY METALS' EFFECTS ON THE RENAL CORTEX OF PREPUBERTAL RATS. Bashkortostan Medical Journal. 2022;17(6):56-61. (In Russ.)
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