THE ROLE AND PLACE OF NICOTINAMIDE COENZYMES IN THE DIAGNOSIS OF INFERTILITY

Abstract

The aim was to study the role of nicotinamide coenzymes (NAD+) in the occurrence of fertility disorders in patients from infertile couples.
Material and methods. To study the concentration of NAD+, patients with idiopathic infertility were divided by age into three equal groups of 15 men. The first group included men aged 20-30 years, the second group included men 31 to 40 years of age, and the third group included men over 40 years of age. The comparison group consisted of 26 fertile donors. The level of NAD+ in ejaculate was determined by colorimetric method (NAD/NADH Cell-Based Assay Kit, Cayman Chemical, USA) according to the manufacturer's instructions.
Results. In patients with infertility of unclear genesis, statistically significant changes in the form of an increase in NAD+ level depending on the age of the patients were established. Negative correlation between the concentration of NAD+ in the ejaculate and sperm quality parameters, including the concentration, sperm motility number, was found.
Conclusion. The ejaculate is assessed as fertile when the NAD+ concentration is in the range of 91-120 nmol/L, regardless of a patient's age. Measurement of NAD+ level enables to reveal early disorders of redox processes in male gametes, pathology of gametogenesis and DNA repair.