THE STUDY OF EXTERNAL AND MICROSCOPIC SINGNS AND QUALITATIVE ANALYSIS OF OXYTROPIS ROSEA BUNGE
Abstract
The purpose of the study presented in the article was to perform macroscopic, microscopic and qualitative analysis of Oxytropis rosea Bunge.
Material and methods. Grass and roots harvested from the wild plants in the Kyrgyz Republic were used for the research.. For qualitative chemical analysis, aqueous, water-alcohol and alcohol extracts with 70%, 96% ethyl alcohol were obtained. Known chemical reactions to flavonoids, polysaccharides, alkaloids, saponins, coumarins, anthracene derivatives, tannins were carried out with the aqueous and alcoholic extracts obtained from this plant. Alkaloids were also studied by thin-layer chromatography. Thinlayer chromatography was performed on Sorbfil aluminium foil analytical plates (PTLC-AF-A) of 100x150 mm in a benzeneethanol system (9:1). The chromatograms were developed with Dragendorf reagent.
Results. Diagnostic morphological and anatomical features of the plant have been established, which can be used to identify raw materials. The presence of groups of biologically active substances such as flavonoids, coumarins, saponins, tannins, alkaloids, has been established. Chemical and chromatographic studies have shown that the raw materials contain alkaloids, however, to establish their group affiliation and structure, a more detailed study using instrumental analysis methods is necessary. These studies can be used to develop regulatory documentation for O. rosea raw materials.
About the Authors
A. Asilbekk
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
S. R. Khasanova
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
N. V. Kudashkina
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
A. D. Muratalieva
Кыргызская государственная медицинская академия имени И.К. Ахунбаева, г. Бишкек
Kyrgyzstan
Kyrgyzstan
T. V. Bulgakov
ФГБОУ ВО «Московский государственный университет имени М.В. Ломоносова»
Russian Federation
Russian Federation
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Review
For citations:
Asilbekk A., Khasanova S.R., Kudashkina N.V., Muratalieva A.D., Bulgakov T.V. THE STUDY OF EXTERNAL AND MICROSCOPIC SINGNS AND QUALITATIVE ANALYSIS OF OXYTROPIS ROSEA BUNGE. Bashkortostan Medical Journal. 2023;18(1):55-59. (In Russ.)
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