THE EFFECT OF 60-DAY ADMINISTRATION OF TARTRAZINE ON THE ULTRASTRUCTURE OF CHROMAFFIN CELLS OF THE ADRENAL MEDULLA IN RATS
Abstract
Objective. To study the ultramicroscopic features of chromaffin cells of the adrenal medulla of rats after 60-day exposure to tartrazine.
Material and methods. The experiment was carried out on 18 white mature rats. One ml of saline solution was administered in- tragastrically to animals of the control group daily for 60 days, while the rats of groups I and II were given an equivalent volume of tartrazine at a dosage of 750 and 1500 mg/kg, respectively. In accordance with the protocol for electron microscopy, the samples of adrenal glands were processed.
Results. In the nucleus of dark and light endocrinocytes, heterochromatin predominated, distributed in the karyoplasm in the form of moderately dense fine-grained accumulations; in the cytoplasm, small secretory granules and a weakly developed rough endoplasmic reticulum were detected in group I. Some mitochondria have destroyed cristae and membrane. In group II, in addition to the changes described above, more areas of the cytoplasm with destroyed contents between the mitochondria were recorded.
Conclusion. Electron-microscopic changes in chromaffin cells of the adrenal medulla, caused by 60-day exposure to tartrazine, indicate a decrease in their functional activity which is directly dependent on the administered dose.
About the Authors
V. N. Morozov
ФГАОУ ВО «Белгородский государственный национальный исследовательский университет»
Russian Federation
Russian Federation
V. I. Luzin
ГУ «Луганский государственный медицинский университет им. Святителя Луки»
Russian Federation
Russian Federation
References
1. Toxicological and Teratogenic Effect of Various Food Additives: An Updated Review / S. Sambu [et al.] // Biomed Res Int. – 2022. – 2022. – P. 6829409.
2. Recent advances in the biodegradation of azo dyes / Y. Shi [et al.] // World J Microbiol Biotechnol. – 2021. – Vol. 37, № 8. – P. 137.
3. Chung K.T. Azo dyes and human health: A review / K.T. Chung // J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. – 2016. – Vol. 34, № 4. – P. 233-261.
4. Research advances in the adverse effects of azo dyes / F.C. Deng [et al.] // Zhonghua Yu Fang Yi Xue Za Zhi. – 2020. – Vol. 54, № 12. – P. 1478-1483.
5. Kanczkowski W. The adrenal gland microenvironment in health, disease and during regeneration / W. Kanczkowski, M. Sue, S.R. Bornstein // Hormones (Athens). – 2017. – Vol. 16, № 3. – P. 251-265.
6. Directive 2010/63/EU of the European Parliament and of the Council of the European Union on the protection of animals used for scientific purposes, complying with the requirements of the European Economic Area. St. Petersburg. – 2012.
7. Reynolds E.S. The use of lead citrate at high pH as an electron-opaque stain in electron microscop / E.S.Reynolds // Journal of cell biology. – 1963. – Vol. 17. – P. 208-212.
8. Kemoklidze K.G. Rat adrenal medulla modular organization / K.G. Kemoklidze, N.A. Tyumina // RUDN Journal of Medicine. – 2022. – Vol. 26, № 3. – P. 259-273.
9. Silva J. Toxicological and ecotoxicological aspects of tartrazine yellow food dye: a literature review / J. Silva, R. Fracacio // Revista brasileira de ciências ambientais. – 2021. – Vol. 56, № 1. – P. 137-151.
Review
For citations:
Morozov V.N., Luzin V.I. THE EFFECT OF 60-DAY ADMINISTRATION OF TARTRAZINE ON THE ULTRASTRUCTURE OF CHROMAFFIN CELLS OF THE ADRENAL MEDULLA IN RATS. Bashkortostan Medical Journal. 2023;18(3):42-45. (In Russ.)
Views:
16
Email this article 
