REORGANIZATION OF PERIPHERAL BLOOD LYMPHOCYTIC PROFILE IN RATS IN THE POST-TRAUMATIC PERIOD
Abstract
The objective of the research was to study the subpopulation composition of lymphocytes in peripheral blood in white rats after mechanical injury of the soft tissues of the thigh.
Material and methods. The study was carried out on white mongrel rats weighing 180-200 g, in which, with the help of a special installation (falling load), a mechanical hip injury was caused simulating high kinetic damage to soft tissues [1]. The object of the impact was the right hind limb.
Results and discussion. The study of the subpopulation composition of lymphocytes in peripheral blood was carried out by im- munophenotyping. After 3 days, suppression of the T- and B-cell immunity link and a change in the relative content of immuno- competent lymphocytic cells (CD3+, CD4+, CD8+, CD19+, CD56+) were determined. After 14 days of the post-traumatic period, partial restoration of the T-cell link and activation of B-lymphocytes occurred.
Conclusion. The maximum severity of changes in blood parameters after 3 days is associated with immunosuppression of all parts of the immune system in the acute period after injury, and partial recovery at a later date (after 14 days) is due to the activation of sanogenesis processes.
Keywords
mechanical hip injury,
blood,
cellular and humoral immunity,
immunophenotyping,
CD3+,
CD4+,
CD8+,
CD19+,
CD56+
About the Authors
A. Kh. Lanicheva
ФГБОУ ВО «Башкирский государственный медицинский университет»
Russian Federation
Russian Federation
V. V. Semchenco
ФГБОУ ВО «Омский государственный аграрный университет имени П.А. Столыпина»
Russian Federation
Russian Federation
E. V. Sosnovskaya
ВО ХМАО-Югры «Ханты-Мансийская государственная медицинская академия»
Russian Federation
Russian Federation
L. A. Sharafutdinova
ФГБОУ ВО «Башкирский государственный медицинский университет»
Russian Federation
Russian Federation
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Review
For citations:
Lanicheva A.Kh., Semchenco V.V., Sosnovskaya E.V., Sharafutdinova L.A. REORGANIZATION OF PERIPHERAL BLOOD LYMPHOCYTIC PROFILE IN RATS IN THE POST-TRAUMATIC PERIOD. Bashkortostan Medical Journal. 2023;18(3):45-50. (In Russ.)
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