MORPHOLOGICAL HETEROGENEITY OF CD34+ CELLS IN EXPERIMENTAL RAT LIVER CIRRHOSIS
Abstract
The object of the work was to study the expression of the CD34 marker by endothelial cells of the intrahepatic vascular bed in experimental rat liver cirrhosis.
Material and methods. Liver cirrhosis in Wistar rats was induced with thioacetamide for 17 weeks. The CD34+ cells were assessed by immunohistochemistry.
Results. In the liver of intact and all experimental rats, endothelial cells of the interlobular arteries, interlobular, central, and sublobular veins expressed the CD34 marker. They had an elongated shape and a rod-shaped dark-colored nucleus. Before the stage of transformation of fibrosis into cirrhosis, there were no CD34+ cells in the sinusoidal capillaries of liver. At the stage of transformation of liver fibrosis into cirrhosis, CD34+ cells of a rounded elongated shape with light rounded elongated nuclei were observed in sinusoidal capillaries. At the same time, among the cells of the lymphoid-histiocytic infiltrate of the connective tissue septa near the portal zones and interlobular bile ducts, islands of rounded CD34+ cells with dark-colored nuclei were observed.
Conclusions. The experiment revealed three morphological phenotypes of CD34+ cells and a slight tendency to increase their area.
About the Author
Ye. I. Lebedeva
УО «Витебский государственный ордена Дружбы народов медицинский университет»
Belarus
Belarus
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Review
For citations:
Lebedeva Ye.I. MORPHOLOGICAL HETEROGENEITY OF CD34+ CELLS IN EXPERIMENTAL RAT LIVER CIRRHOSIS. Bashkortostan Medical Journal. 2023;18(3):50-54. (In Russ.)
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