DEVELOPMENT OF A METHOD OF STANDARTIZATION OF THE LEAVES OF PUNICA GRANATUM L. ACCORDING TO THE CONTENT OF FLAVONOIDS

Objective of the research. Development of a method for standardization of the leaves of common pomegranate (Punica gran-atum L.) according to the content of flavonoids.

Material and methods. The object of the study was the leaves of the common pomegranate (Punica granatum L.) harvested during its flowering in 2020-2022 on the territory of the Republic of Azerbaijan.
The spectral analysis of alcohol extracts was carried out in the range from 220 to 450 nm in order to establish the dominant flavonoid. For the quantitative determination of flavonoids, the method of differential spectrophotometry in the UV and visible regions was used.

Results. In the course of the development of the method, it was established that the optimal parameters for the extraction of flavonoids from the Punica granatum leaves are: the extractant - ethyl alcohol 95%, the correspondence of the mass of raw materials and the amount of extractant - 1:100, the multiplicity of extraction - single, the optimal particle size of raw materials - 1 mm, the extraction time - 30 minutes, the concentration of aluminium chloride solution of 1% in ethyl alcohol 70%, the amount of the added complexing agent - 5 ml, the reaction time with the complexing agent - 40 minutes.

Conclusions. Spectral characteristics of alcohol extracts from leaves of Punica granatum were studied, indicating the dominance of apigenin. A method of standardization of Punica granatum leaves on the quantitative content of flavonoids in terms of apigenin is proposed

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