DEVELOPMENT OF CRITERIA FOR AUTHENTICITY AND QUANTITATIVE DETERMINATION OF THE AMOUNT OF SAPONINS IN GYNOSTEMMA PENTAPHYLLUM HERB

The purpose of the work: development of criteria for the authenticity of raw materials and the quantitative determination of the amount of saponins in Gynostemma pentaphyllum herb.

Material and methods. The object of study was G. pentaphyllum (Thunb.) Makino herb harvested in the flowering phase from 2017 to 2020 on the territory of the Republic of Bashkortostan from a cultivated species. The determination of the authenticity of raw materials and the quantitative content of the amount of triterpene saponins in G. pentaphyllum was carried out by the spectrophotometric method based on the reaction with sulfuric acid.

Results. The criterion for the authenticity of saponins in Gynostemma pentaphyllum is a qualitative reaction with concentrated sulfuric acid followed by measurement of the UV spectra of the obtained complexes. The development of the quantitative determination of the amount of saponins was carried out by selecting the optimal extraction conditions: the type of extractant, the ratio of the weight of the raw material and the volume of the extractant, the extraction time, the degree of grinding of the raw material and the reaction time with concentrated sulfuric acid. In accordance with the selected conditions, the amount of saponins in G. pentaphyllum herb averages 17.24±0.07%.

Conclusions. Authenticity criteria and a method for quantifying the amount of saponins in raw materials in terms of β-escin, which is easily reproducible, accessible and not time-consuming, have been developed. The technique allows to evaluate the quality of Gynostemma pentaphyllum herb by the content of one of the groups of the main bioactive compounds – saponins.

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