COMPARATIVE ANALYSIS OF THE ANTIDEPRESSANT ACTIVITY OF THYMUS MARSCHALLIANUS AND THYMUS SERPYLLUM EXTRACTS USING THE PORSOLT TEST
Abstract
The aim is to study the effect of extracts of Thymus marschallianus Willd. and Thymus serpyllum L. on the antidepressant activity of mice in the Porsolt test.
Material and methods. The evaluation of the antidepressant activity of animals was carried out using the Porsolt test on the 1st, 8th, 15th and 21st days of the experiment. The mice were divided into 6 groups – a control group and five experimental ones (6 animals in each group) receiving intragastric amitriptyline (10 mg/kg), alcoholic extract of Thymus serpyllum, water extract of Thymus serpyllum, alcoholic extract of Thymus marschallianus, water extract of Thymus marschallianus (the concentration of the thick extract was 100 mg/kg) during 21 days of the experiment. The control group received drinking water in an equivalent amount.
Results. Alcoholic and water extracts of Thymus marschallianus and Thymus serpyllum at a dose of 100 mg/kg did not show a statistically significant increase in the time of active swimming or a decrease in immobilization during the 21 days of the experiment compared to the group of control mice. On the 15th and 21st days of the study, the immobilization time in the experimental groups was statistically significantly higher compared to the group of animals treated with amitriptyline (10 mg/kg).
Conclusion. Alcoholic and water extracts of Thymus marschallianus and Thymus serpyllum at a dose of 100 mg/kg showed no antidepressant activity during the 21-day experiment.
About the Authors
A. S. Sheremetyeva
ФГБОУ ВО «Саратовский государственный медицинский университет имени В.И. Разумовского» Минздрава России
Russian Federation
Russian Federation
A. Yu. Karetnikova
ФГБОУ ВО «Саратовский государственный медицинский университет имени В.И. Разумовского» Минздрава России
Russian Federation
Russian Federation
N. A. Durnova
ФГБОУ ВО «Саратовский государственный медицинский университет имени В.И. Разумовского» Минздрава России
Russian Federation
Russian Federation
References
1. Porsolt RD., Anton G., Blavet N., Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol. 1978;47(4):379–391 (In Engl) DOI: 10.1016/0014-2999(78)90118-8
2. Abo Kh.M, Primysheva E.N. Depressiya, rezistentnaya k terapii: novye opredeleniya i podkhody k lecheniyu (Therapy-resistant depression: new definitions and treatment approaches). Nauchnyi ehlektronnyi zhurnal Meridian. 2021;5(58):84–86 (In Russ)
3. Mazo G.E., Gorbachev S.E., Petrova N.N. Modern strategies of diagnostic and management for antidepressant nonresponse). Vestnik of Saint Petersburg university. Medicine. 2008;(2):87–96 (In Russ)
4. Kurkin V.A., Kurkina, A.V., Zaitseva, E. N. [et al.] Neurotropic and diuretic activity of thick extract of the blood-red hawthorn’s fruits. Bulletin of siberian medicine. 2015;14(3):18–22 (In Russ).
5. Barkin I.M., Fedorova Yu.S., Kulpin P.V., Suslov N.I. Research of psychotropic properties of extracts of some plants. National health. 2020;(1):30–33 (In Russ).
6. Husain GM., Chatterjee SS., Singh PN., Kumar V. Beneficial effect of Hypericum perforatum on depression and anxiety in a type 2 diabetic rat model. Acta Polon. Pharmaceut. 2011;68(6):913–918 (In Engl).
7. Panossian A., Nikoyan N., Ohanyan N. [et al.] Comparative study of Rhodiola preparations on behavioral despair of rats. Phytomedicine. 2008;15(1-2):84–91 (In Engl) DOI: 10.1016/j.phymed.2007.10.003.
8. Morteza-Semnani K., Mahmoudi M., Riahi G. Effects of essential oils and extracts from certain Thymus. Species on swimming performance in mice. Pharmaceutical Biology. 2007;45(6):464–467 (In Engl) DOI: 10.1080/13880200701389177
9. Doosti MH., Ahmadi K., Fasihi-Ramandi M. The effect of ethanolic extract of Thymus kotschyanus on cancer cell growth in vitro and depression-like behavior in the mouse. Journal of traditional and complementary medicine. 2018;8(1):89–94 (In Engl) DOI: 10.1016/j.jtcme.2017.03.003.
10. Karetnikova A.Yu., Sheremetyeva A.S., Moskvina A.O., Durnova N.A. Comparative study of the antidepressant activity of Thymus marschallianus Willd. and Thymus serpyllum L. extracts in the tail suspension test on outbred male mice. Traditsionnaya meditsina. 2022;3(69):52–58 (In Russ) DOI: 10.54296/18186173_2022_3_52.
11. Fedorov AA, Menitskii YL. Flora evropeiskoi chasti SSSR (Flora of the European part of the USSR). Leningrad: Nauka, 1978:259 (In Russ).
12. Gosudarstvennaya farmakopeya Rossiiskoi Federatsii. XIV izdanie (State Pharmacopoeia of the Russian Federation. XIV edition) [Ehlektronnyi resurs]. URL: https://femb.ru/record/pharmacopea14
13. Sposob poluchenija suhogo jekstrakta iz rastitel'nogo syr'ja, obladajushhego biologicheskoj aktivnost'ju (A method for obtaining a dry extract from plant raw materials with biological activity): pat. № 2482863 Ros. Federacija; zajavl. 15.02.2012; opubl.: 27.05.2013. Bjul. № 15. 11 s. (In Russ).
14. Sheremetyeva A.S., Durnova N.A., Raikova S.V. Research of antimicrobial activity water-alcohol extract Thymus marchallianus. Sovremennye tendentsii razvitiya tekhnologii zdorov'esberezheniya. Moskva: FGBNU VILAR, 2019.2019:509–514. (In Russ).
15. Sheremetyeva A.S., Shapoval O.G., Frolkova A.V., Durnova N.A. Antimicrobial activity of extracts of Thymus marschallianus and Thymus serpyllum against clinical strains of microorganisms. Traditsionnaya meditsina. 2021;3(66):27–32. (In Russ). DOI: 10.54296/18186173_2021_3_27
16. Sheremetyeva A.S., Napsheva A.M., Durnova N.A. Antitumor activity in vivo of aqueous and alcoholic extracts of Thymus marschallianus Willd. Pharmacy & Pharmacology. 2021;9(6):476–484 (In Russ). DOI: 10.19163/2307-9266-2021-9-6-476-484.
17. Sheremetyeva A.S., Karetnikova A.Yu., Durnova N.A. [et al.] Comparative study of the effect of Thymus marschallianus Willd. extracts on the cognitive functions of mice in the radial maze. Reviews on clinical pharmacology and drug therapy. 2021;19(4):443–449 (In Russ) DOI: 10.17816/RCF194443-449.
18. Karkischenko V.N., Kapanadze G.D., Dengina S.E. [et al.] Working out of a technique for physical endurance of small laboratory animals for studying of different medicine. Biomedicine. 2011; (1):72-74 (In Russ).
19. Frolova G.A Comparative ethological characteristics of males and females of white rats in porsolt test in blocking receptors of sex hormone. Proceeding of Voronezh State University. Series: Chemistry. Biology. Pharmacy. 2016(4):110-116 (In Russ).
20. Voronkov A.V., Gerashchenko A.D., Pozdnyakov D. I. [et al.] Effects of various aversive environments on oxygen consumption of muscle and blood in mice under conditions of the "forced swimming" test. Pharmacy & Pharmacology. 2019;7(3):148-157 (In Russ) DOI: 10.19163/2307-9266-2019-7-3-148-157.
21. Grzhibovsky A. M., Ivanov S. V., Gorbatova M. A. Analysis of quantitative data in three or more independent groups using statistica and spss software: parametric and non-parametric tests. Science & Healthcare. 2016;(4):5-37 (In Russ).
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For citations:
Sheremetyeva A.S., Karetnikova A.Yu., Durnova N.A. COMPARATIVE ANALYSIS OF THE ANTIDEPRESSANT ACTIVITY OF THYMUS MARSCHALLIANUS AND THYMUS SERPYLLUM EXTRACTS USING THE PORSOLT TEST. Bashkortostan Medical Journal. 2023;18(4):66-71. (In Russ.)
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