Qualitative and quantitative structural transformations of the thyroid gland of rats after 60 days of intoxication with toluene
Abstract
Objective is to establish qualitative and quantitative changes in the microstructure of the thyroid gland of rats after 60 days of intoxication of their bodies with toluene.
Material and methods. An experimental study was carried out on 60 male rats of the reproductive period, divided into two groups: 1 – for 60 days it was seeded with toluene vapors at a concentration of 500 mg/m3, 2nd served as a control. After completion of the 60-days intoxication of the body with toluene, the thyroid gland was studied on days 1, 7, 15, 30 and 60. The method of light microscopy made it possible to evaluate qualitative, and the method of histomorphometry – quantitative changes occurring in the thyroid gland.
Results. Qualitative transformation included functional disparity of lobules, vascular fullness, swelling and dilatation of stromal layers, topographic heterogeneity of follicles, flattening of follicular epithelium and thyrocytes nuclei, activation of desquamative processes, densification and sharp eosinophilicity of colloid, absence of vacuoles of resorption. Quantitative statistically significant changes were maximally expressed on the 1-st day of observation: a decrease in the height of thyrocytes by 14.70% and an increase in the diameter of follicles by 12.07%, the number of thyrocytes by 10.72%, the colloid accumulation index by 30.04%.
Conclusion. Microscopic examination of the thyroid gland of rats exposed to toluene showed polymorphism of criteria for the morphofunctional state of the organ with a predominance of signs of its hypofunction during all observation periods.
About the Authors
K. A. Fomina
ФГБОУ ВО «Луганский государственный медицинский университет имени Святителя Луки» Минздрава России
Russian Federation
Russian Federation
V. I. Berov
ФГБОУ ВО «Луганский государственный медицинский университет имени Святителя Луки» Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Fomina K.A., Berov V.I. Qualitative and quantitative structural transformations of the thyroid gland of rats after 60 days of intoxication with toluene. Bashkortostan Medical Journal. 2024;19(4):33-37. (In Russ.)
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