EVALUATION OF THE ROLE OF THE COLONIC MICROBIOME IN THE PATHOGENESIS OF IRON DEFICIENCY ANAEMIA IN YOUNG AND MIDDLE-AGED WOMEN

Abstract

Objective. To analyze the relationship between colonic dysbiosis and iron deficiency anemia in 120 women aged 18 to 49 years old.

Material and methods. The following materials and methods were used: colonoflor-16 method combined with real-time polymerase chain reaction (PCR) was used to assess colonic dysbiosis. Study participants suffered from mild iron deficiency anemia, as demonstrated by hemoglobin levels between 110 and 119 g/L, serum iron levels below 10 g/L, and ferritin levels below 30 μg/L. The mean age of the participants was 34 years old and BMI was 23.11±3.47 kg. 58 women (48.3%) had a history of childbirth. More than half of the women in the study (54.17%) had previously taken iron preparations.

Results. A significant change in the microbiota of the examined patients was revealed - 88.9%, which was characterised by a clinically significant decrease in the commensal microorganisms Lactobacillus spp. and Bifidobacterium spp. and a significant increase in the titres of Klebsiella pneumoniae, Klebsiella oxytoca, Candida spp., Staphylococcus aureus, Clostridium perfringens, Citrobacter spp. and Enterobacter spp. In addition, there was an increase in the coefficient of anaerobic imbalance characteristic of inflammatory bowel diseases and autoimmune conditions against the background of mild iron deficiency. The detected disturbances in the total number of microbial cells of the large intestine were accompanied by an increase in the coefficient of anaerobic imbalance.

Conclusion. The observed changes confirm the research data on the influence of large intestinal dysbiosis on the pathogenesis of iron deficiency anemia.