OPTIMIZATION OF EXTRACTION PROCESSES OF BIOLOGICALLY ACTIVE SUBSTANCES FROM NATURAL RAW MATERIALS BASED ON THE QBD PRINCIPLE «QUALITY BY DESIGN»

Abstract

Objective. To investigate the influence of key technological parameters on the process of glycyrrhizic acid extraction from licorice roots (Glycyrrhiza glabra L.) through a series of single-factor experiments and to develop a mathematical model of the process.

Material and methods. Extraction was carried out by the maceration method. Quantitative determination of glycyrrhizic acid was performed spectrophotometrically. Extraction model parameters were calculated by nonlinear regression in Minitab v.20 software.

Results. The following factors were found to have a significant effect on the extraction efficiency: temperature (in the range of 25-100 °C), solid-liquid ratio (10-30 ml/g), raw material particle size (up to 5 mm), process time (up to 30 minutes), addition of NaHCO3 (up to 1%) and stirring (up to 100 rpm). The model describing the dependence of extraction efficiency on key variables (time, temperature, solid-liquid ratio) was developed.

Conclusions. The influence of process parameters on the extraction of glycyrrhizic acid from licorice roots was studied, critical process factors were determined. A mathematical model suitable for process optimization and scaling within the QbD concept was constructed.