OBTAINING HUMAN INDUCED PLURIPOTENT STEM CELLS FROM CORNEAL KERATOCYTES
Abstract
Lack of donor material and immune rejection are an acute problem of existing methods of keratoplasty. Modern cellular technology can be an effective solution. In our work, we consider human corneal keratocytes as an accessible source of induced pluripotent stem cells.Human keratocytes isolated from the donor cornea were subjected to episomal reprogramming. Within a day the colony of ker-atocytes was affected by a number of reprogramming factors: Oct4, Sox2, Klf4, Lin28, L-Myc and p53. After several phases of keratocytes division, cell foci similar in their characteristics to pluripotent stem cells were identified. The derived induced pluripotent stem cells were mechanically isolated, passaged and verified by immunofluorescence analysis.
About the Authors
T. I. Bikkuzin
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
V. N. Pavlov
ФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
Y. . She
Harbin Medical University
Russian Federation
Russian Federation
H. . Zhang
Harbin Medical University
Russian Federation
Russian Federation
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Review
For citations:
Bikkuzin T.I., Pavlov V.N., She Y., Zhang H. OBTAINING HUMAN INDUCED PLURIPOTENT STEM CELLS FROM CORNEAL KERATOCYTES. Bashkortostan Medical Journal. 2018;13(4):31-34. (In Russ.)
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