IN VITRO ANALYSIS OF PROLIFERATIVE ACTIVITY OF CELLS ON THE COLLAGEN 3D MATRIX FOR REGENERATION OF SOFT TISSUES OF THE ORAL CAVITY

Objective: a comparative analysis of the combined use of preparations of activated plasma, hyaluronic acid and collagen 3Dmatrix in vitro.Materials and methods. For the experiment, fibroblasts from the lungs of a human embryo were used. The proliferative activity of fibroblasts was evaluated in the presence of activated plasma, the preparation of hyaluronic acid and the 3D collagen matrix Fibromatrix for the regeneration of soft tissues of the oral cavity. Evaluation of cytotoxicity and proliferative activity was estimated bycolorimetric MTT test in 1, 3 and 7 days. The results were recorded using microscopic examination and photographing in transmit-ted light.Results. It was found, that stable active growth of fibroblasts was observed with the combined use of collagen 3D matrix and activated plasma. A preparation based on hyaluronic acid promotes the proliferation of human fibroblasts, but its activity decreases, inhibiting cell growth by 7th day.Conclusion. The presented results demonstrate the prospects of using a combination of autogenous platelet plasma and 3D collagen matrix for the regeneration of soft tissues of the oral cavity in clinical practice.

регенерация, пролиферация фибробластов, рецессия десны, хирургическое лечение десневой рецессии, тромбоцитарная аутогенная плазма, коллагеновый матрикс, гиалуроновая кислота, regeneration, fibroblast proliferation, gingival recession, surgical treatment of gingival recession, platelet autologous plasma, collagen matrix, hyaluronic acid