ANATOMICAL AND MORPHOMETRIC STUDIES OF THE THYROID GLAND UNDER THE ACTION OF SPA AND PHYSICAL FACTORS

Abstract

The purpose of the study was to assess the effects of Taldinskyi spring water on cytoonstructionl of the thyroid gland.Material and methods. The work was performed on 20 white male rats weighing 120-140 g. The animals were divided into 2 groups: control and experimental. Animals of the experimental group received 10 baths of water containing about 450 mg / l of hydrogen sulfide (water temperature 36-37°C). Taking baths was carried out in conditions close to medical. Macro-and microscopicmeasurements were performed. Under a light microscope, the diameter of the follicles, the height of the follicular epithelium, theaverage area of the thyrocyte nuclei were measured, and the brown index was calculated, reflecting the ratio of the diameter of the follicle cavity to the height of the thyrocyte (d/2h). The concentration of thyroid hormones in the blood plasma was determined by radioimmune method (set "RIO-T3-PG" HOP IBOH AN Belarus).Results. In experimental rats, there was a large variability in follicle diameters. A 14% reduction in the size of follicle diameters was revealed. The average diameter of thyrocyte nuclei increased by 1.3 times. It was found that the relative volume of lymphocytic infiltration increased by 1.7 times, compared with the control group. The content of thyroxine in the blood serum increased by 83% in comparison with the indicators in the intact group; T3 - by 28%; and TTG - by 52% (P≤0.05). A number of morphological changes are associated with the influence of specific (temperature, hydrostatic) factors, and the hormonal activity of the thyroid gland is largely determined by the nature, character and intensity of the impact of mineral waters.