INFLUENCE OF G1082A IL-10 GENE POLYMORPHISM ON THE COURSE OF ACUTE INTESTINAL INFECTIONS

Abstract

In the development, course and outcomes of acute intestinal infections, a significant role is played by the polymorphism of genes responsible for the induction of the synthesis of inflammatory molecules.Purpose of the work: to identify the features of the clinical picture depending on the polymorphism of the G1082A IL-10 gene and its effect on the induction of IL-10 synthesis.Material and methods. 108 people diagnosed with acute intestinal infection were studied. In the blood serum, the level of IL-10was determined using the test systems of «Vector-Best», Novosibirsk. Determination of the polymorphism of the G1082A IL-10 gene was carried out by PCR using primers of «Litekh» LLC (St. Petersburg). Genomic DNA was analyzed. Statistical data processing was carried out using electronic programs Microsoft Excel 2010, Statistica 6.0.Results. In all the subjects, the predominance of the dominant allele G of the G1082A locus of the IL-10 gene was found. At the same time, in patients with acute intestinal infection, who are owners of the hypoproductive allele A in the -AA1082 variant of the IL-10 gene, a significant decrease in the expression of interleukin 10 was noted, which affected the course of the gastrointestinal tract lesion syndrome.Conclusions. The presence of the IL-10 G1082A gene polymorphism, without affecting the risk of developing acute intestinal infections, affects the induction of IL-10 expression and the clinical picture of the disease.