DEVELOPMENT OF A METHOD FOR QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF GYNOSTEMMA PENTAPHYLLUM

The article is devoted to the development of a technique for the quantitative determination of flavonoids in the herb of Gynostemma pentaphyllum (Thunb.) Makino by the spectrophotometric method in terms of rutin. The raw materials of the Gynostemma pentaphyllum were harvested at experimental sites for several years.Preliminary spectral studies of water-alcohol extracts from the herb Gynostemma pentaphyllum on 70% ethyl alcohol showed absorption maxima close to the absorption maxima of rutin. Therefore, in the quantitative analysis of flavonoids in the gynostemma, the conversion was carried out to rutin.When developing the technique, the main extraction conditions were selected: the type of extractant, the ratio of the mass of the raw material and the volume of the extractant, the degree of grinding of the raw material, the multiplicity and time of extraction. The conditions of the analysis were also investigated: the volume of aluminum chloride alcohol solution, reaction time and stability of the complex.According to the results obtained, the content of flavonoids in the studied raw materials was 3.76 ± 0.04%. It is proposed to include in the regulatory document for Gynostemma pentaphyllum raw materials the developed methodology with a quality indicator - the content of the sum of flavonoids is not less than 2%.

raw materials, Gynostemma pentaphyllum, rutin, flavonoids, aluminum chloride, spectrophotometry

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