EXPERIENCE IN USING AND EVALUATING THE EFFECTIVENESS OF A COMMERCIAL DNA EXTRACTION KIT FROM WHOLE THAWED BLOOD OF PATIENTS WITH BRONCHIAL ASTHMA

The process of isolation and purification of DNA from biological material is one of the most important and key stages of molecular genetic research on the quality of execution, which largely depends on the success and sensitivity of all its subsequent stages, as well as the correct and reliable initial result.The data on the experience of using the column filtration method by a commercial set of ExtractDNA Blood reagents (CJSC«Eurogen», Russia) for extracting high-concentration DNA extract and purification from samples of whole thawed blood of 169 patients suitable for subsequent genotyping are presented.Material and methods. The effectiveness of the DNA extraction technique we have chosen was evaluated by spectrophotometric indicators of the concentration and purity of samples obtained during the work from impurities, traces of preservatives and substances inhibiting its analysis.Results and conclusions. Taking into account the ease of use of this commercial kit, the safety of the components included in its composition, as well as sufficient spectrophotometric data for subsequent analysis: average purity (A260/A280) = 1,896 and average concentration (C) = 56.87 ng/ml of isolated DNA from thawed blood samples, it can be concluded that the method of column filtration chosen by us with a commercial set of ExtractDNA Blood reagents (CJSC «Eurogen», Russia) is optimally suitable for DNA extraction.

DNA extraction, whole thawed blood, concentration and purity of DNA, spectrophotometric indicators, a set of Extract DNA Blood reagents