DEVELOPMENT OF A METHOD OF QUANTITATIVE DETERMINATION OF THE CONTENT OF FLAVONOIDS IN FLOWERS OF TRIPLEUROSPERMUM PERFORATUM (L.) SCH.BIP

In the officinal medicine of Russia and Poland, the Tripleurospermum perforatum (L.) Sch.Bip. is considered an admixture to matricary. However, in folk medicine, the Tripleurospermum perforatum (L.) Sch.Bip. used as an emollient, anti-inflammatory, analgesic and antispasmodic. Antibacterial and antimycotic properties have been established for the raw material of the Tripleurospermum perforatum. It should be noted that there is not enough data in the literature on the analysis of the chemical composition of the Tripleurospermum perforatum (L.) Sch.Bip., which grows on the territory of the Russian Federation. Tripleurospermum perforatum (L.) Sch.Bip. has a sufficient raw material base and habitat, which makes it possible to recommend its raw materials as officinal provided there are developed quality control methods allowing to determine the dominant group of biologically active compounds, which,according to the literature and experimental data, are flavonoids.The aim was to develop a method of analyzing the amount of flavonoids in the flowers of the Tripleurospermum perforatum(L.) Sch.Bip. and validation of this method.Material and methods. The object of the study was the air-dried flowers of the Tripleurospermum perforatum (L.) Sch.Bip. The analysis of the total content of flavonoids in the flowers of the Tripleurospermum perforatum (L.) Sch.Bip. was carried out with spectrophotometry.Results. The influence of the concentration of the extractant, the time of extraction, the multiplicity of extraction of flavonoids have been considered, the conditions for the reaction of complex formation of flavonoids with aluminum chloride have been determined. The optimal conditions for analysis have been established. The method has been validated according to the criteria: specificity, linearity, correctness, precision.

flavonoids, Tripleurospérmum perforatum, spectrophotometry, extraction, multiplicity of extraction, validation