CONTENT OF PHENOLIC COMPOUNDS IN PLANTS

Abstract

For the quantitative determination of phenolic compounds, a technique based on the reaction of polyphenols with the Folin- Ciocalteu reagent is widely used.The purpose of this work was to determine the content of substances of phenolic nature in plant objects. We have selected samples containing significant amounts of flavonoids, tannins, phenolic acids.Material and methods. For the study, extracts from the objects under study were used, obtained by extracting raw materials with ethyl alcohol 50%, 70%, 90% and purified water. Calculation of phenolic compounds was carried out according to the calibration curve of the interaction of gallic acid with the Folin-Ciocalteu reagent.Results. The highest content of the sum of phenolic compounds - 25,2±0,3% is observed in the extract from the grass Lysimachia punctata, obtained by extraction with 50% ethyl alcohol. The stabilization of the obtained complexes was observed within 45-60 min. The spectrophotometric method made it possible to estimate the content of the total polyphenolic compounds in plant objects in terms of gallic acid with the Folin-Ciocalteu reagent.